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Primary Antibodies
AATF Antibody
Background
AATF (apoptosis antagonizing transcription factor) was initially discovered as an interaction partner of ZIP kinase (ZIPK) (1), a member of death-associated protein (DAP) kinase family of pro-apoptotic serine/threonine kinases (2). AATF is a phosphoprotein containing an acidic region and a putative leucine zipper domain and nuclear localization signal, features which are typical of transcription factors. AATF inhibits the ZIPK-mediated pro-apoptotic pathway and may activate other anti-apoptotic pathways (3). Recently, it has also been shown to protect neural cells against oxidative damage induced by amyloid b-peptide and to inhibit aberrant production of the b-peptide by interacting with Par-4 (prostate apoptosis response-4), another pro-apoptotic leucine zipper protein that is associated with neuronal degeneration in Alzheimer’s disease (AD) (4,5), suggesting that AATF may have potential therapeutic applications in both familial and sporadic forms of AD.
Additional Names
AATF, Apoptosis antagonizing transcription factor, Che-1
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Description
Left: Western blot analysis of AATF in human small intestine cell lysate with AATF antibody at (A) 0.5, (B) 1 and (C) 2 μg/ml.
Below: Immunohistochemistry of AATF in human small intestine tissue with AATF antibody at 10 μg/ml.
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Other Product Images
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Source
AATF antibody was raised against a 12 amino acid peptide from near the carboxy terminus of human AATF.
Purification
Immunoaffinity purified IgG
Clonality / Clone
This is a polyclonal antibody.
Host
AATF antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human AATF Peptide (Cat. No. 3039P)
Application
AATF antibody can be used for the detection of AATF by Western blot at 0.5 – 1 μg/ml. (Optimal dilution should be determined by user). Human small intestine cell lysate can be used as positive control. AATF antibody is human and mouse reactive. This product is for research use only.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3039P - AATF Peptide
Storage
AATF antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1308 - Human Small Intestine Tissue Lysate
Species Reactivity
H, M
Protein GI Number
7657013
Protein Accession Number
NP_036270
Short Description
An anti-apoptotic transcription factor
This product belongs to the following categories:
Related Products
References- AATF antibody can be stored at 4˚C, stable for one year. Page G, Lodige I, Kogel D et al. AATF, a novel transcription factor that interacts with Dlk/ZIP kinase and interferes with apoptosis. FEBS Lett. 1999; 462:187-191.
- Kawai T, Matsumoto M, Takeda K, et al. ZIP kinase, a novel serine/threonine kinase which mediates apoptosis. Mol. Cell Biol. 1998; 18:1642-51.
- Lindfors K, Halttunen T, Huotari P, et al. Identification of novel transcription factor-like gene from human intestinal cells. Biochem. Biophys. Res. Commun. 2000; 276:660-6.
- Xie J and Guo Q. AATF protects neural cells against oxidative damage induced by amyloid b-peptide. Neurobiol. of Dis. 2004; 16:150-7.
Datasheet 08-02W
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