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Primary Antibodies
5-Methylcytosine Mouse Monoclonal (33D3)
Background
Methylation of DNA cytosine bases plays important roles in the regulation of gene transcription. It has been estimated that 60 to 90% of the cytosines in CpG dinucleotides are methylated. An increase in methyl-CpG correlates with transcriptional silencing for the whole chromosome especially in developmentally regulated genes. Methylation is believed to lead to transcriptional silencing by multiple mechanisms including alteration of transcription factor binding to promoters and alteration in chromatin structure. Clone number 33D3 is useful in several applications including: ELISA (0.1 μg/ml), Flow Cytometry (1 μg/ml), Cryo Sections (5-10 μg/ml),Paraffin Sections (5-10 μg/ml), and RIA (0.1 μg/ml).
Purification
Mouse IgG1 is purified by Protein A affinity chromatography method.
Clonality / Clone
This is a monoclonal antibody. 33D3
Host
5-Methylcytosine Mouse Monoclonal (33D3) was raised in mouse.
Please use anti-mouse IgG secondary antibodies.
Immunogen
5-Methylcytosine (33D3) monoclonal antibody was (33 D3) was made against 5-methylcytosine conjugated to ovalbumin.
Application
Tested Application
E, IHC, FS, RIA
Storage
5-Methylcytosine Mouse Monoclonal (33D3) is supplied in PBS buffer containing 0.02% sodium azide. For longer periods of storage, store at -20ºC. Avoid repeat freeze-thaw cycle.
Species Reactivity
H, M, R, D
Short Description
Anti-5-Methylcytosine
This product belongs to the following categories:
References- Fraga, MF., et al., (2004) Cancer Res. 64(16), 5527-34
Datasheet 0802-W
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