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Primary Antibodies
4E-T Antibody
Background
4E-T Antibody.
Additional Names
EIF4ENIF1, 4E-T, Clast4, FLJ21601, 2610509L04Rik, eukaryotic translation initiation factor 4E nuclear import factor 1, eIF4E-transporter
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Description
Left: Western blot analysis of 4E-T in 293 lysate (RIPA buffer, 35μg total protein per lane) using 4E-T antibody (0.25μg/mL).
Below: Immunohistochemistry staining of human pancreas using 4E-T antibody at 5μg/ml.
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Other Product Images
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Source
4E-T antibody was raised against a synthetic peptide of 4E-T.
Purification
4E-T antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
4E-T antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Peptide ELISA: Antibody detection limit dilution >1/32,000. Western Blot: Approx 140-150kDa band observed in 293 lysates (predicted size of approx. 108kDa according to NP_062817.1, however our observation agrees with that of Dostie et al (see below) ). Recommended for use at 0.25-0.5μg/ml. Immunohistochemistry: In paraffin embedded Human Pancreas shows strong staining of cytoplasm in acinar cells. Recommended concentration 5-10μg/ml.
Tested Application
E, WB, IHC
Buffer
4E-T antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Blocking Peptide
Cat. No. 45-177P - 4E-T Peptide
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Positive Control
- Cat. No. 1210 - 293 Cell Lysate
Species Reactivity
H, M, R
Protein Accession Number
NP_062817.1
Short Description
4E-T / EIF4ENIF1
This product belongs to the following categories:
Related Products
References- Dostie J, Ferraiuolo M, Pause A, Adam SA, Sonenberg N. A novel shuttling protein, 4E-T, mediates the nuclear import of the mRNA 5' cap-binding protein, eIF4E. EMBO J. 2000 Jun 15;19(12):3142-56.
Datasheet 09-01W
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