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Primary Antibodies
4E-BP1 Antibody
Background
The translation of mRNA in eukaryotic cells is regulated by the presence of amino acids through multiple mechanisms (reviewed in 1). One such mechanism involves the evolutionarily conserved serine/threonine kinase TOR (Target of rapamycin, also known as mTOR), which regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors (reviewed in 2). One downstream target of TOR is the eukaryotic initiation factor 4E binding protein 1 (4E-BP1) whose phosphorylation prevents its association with eIF4E, preferentially stimulating translation of mRNAs containing long, highly structured 5’-UTRs (1). Rapamycin inhibits TOR resulting in reduced cell growth and reduced rates of cell cycle and cell proliferation (reviewed in 3), at least in part by inhibiting the activity of TOR towards 4E-BP1.
Additional Names
4E-BP1, initiation factor 4E binding protein 1
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Description
Left: Western blot analysis of 4E-BP1 in 3T3 cell lysate with 4E-BP1 antibody at (A) 2.5, (B) 5 and (C) 10 μg/ml.
Below: Immunocytochemistry of 4E-BP1 in 3T3 cells with 4E-BP1 antibody at 2 μg/ml.
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Other Product Images
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Source
4E-BP1 antibody was raised against a 14 amino acid peptide from near the carboxy-terminus of human 4E-BP1.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
4E-BP1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human 4E-BP1 / initiation factor 4E binding protein 1 Peptide (Cat. No. 3513P)
Application
4E-BP1 antibody can be used for the detection of 4E-BP1 by Western blot at 2.5 – 5 μg/ml.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3513P - 4E-BP1 Peptide
Storage
4E-BP1 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1212 - 3T3 Cell Lysate
Species Reactivity
H, M
Protein GI Number
4758258
Protein Accession Number
NP_004086
Short Description
a eukaryotic translation initiation factor
This product belongs to the following categories:
Related Products
References- Shah OJ, Anthony JC, Kimball SR, et al. 4E-BP1 and S6K1: translational integration sites for nutritional and hormonal information in muscle. Am. J. Physiol. Endocrinol. Metab. 2000; 279:E715-29.
- Shamji AF, Ngheim P, and Schreiber SL. Integration of growth factor and nutrient signaling: implications for cancer biology. Mol. Cell 2003; 12:271-80.
Datasheet 08-02W
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