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Primary Antibodies
4E-BP1 Antibody
Background
Eukaryotic initiation factor 4E binding protein 1 (4E-BP1), also known as PHAS, is a ~20 kDa member of a family of eIF4E-binding proteins whose binding affinity to eIF4E is regulated by its phosphorylation. It inhibits cap-dependent translation by binding to eIF4E on the same site that overlaps the binding site for eIF4G, preventing its binding to the latter and eventually leading to an increase in mRNA translation. The phosphorylation of 4E-BP1 is critical in determining cell fate by controlling translation initiation and apoptotic potency. 4E-BP1 is hyperphosphorylated in response to several external stimuli including hormones, growth factors, mitogens, cytokines and G-protein–coupled receptors and in response to stress conditions including nutrient deprivation. The phosphorylation of 4E-BP1 increases in response to activated phosphoinositol 3’-kinase (PI-3K) or its downstream effector Akt/PKB. 4E-BP1 is believed to mediate PI-3K and FRAP/mTOR signaling and is phosphorylated on at least six serine and threonine sites (Thr 37, Thr 46, Ser 65, Thr 70, Ser 83, and Ser 112). The phosphorylation of these sites is believed to occur in an orderly fashion where phosphorylation of threonine 37 and 46 by FRAP/mTOR is a priming step for subsequent phosphorylation of 4E-BP1 at the carboxy-terminal sites.
Source
4E-BP1 antibody was produced against recombinant human 4E-BP1 protein expressed in E. coli.
Clonality / Clone
This is a monoclonal antibody.
Host
4E-BP1 antibody was raised in mouse.
Please use anti-mouse secondary antibodies.
Specificity
Human, mouse and rat.
Application
4E-BP1antibody is suitable for use in Western blotting.
Tested Application
WB
Buffer
Purified immunoglobulin in phosphate buffered saline, pH 7.2, with 1% bovine serum albumin.
Storage
Store at 2-8˚C. For long term storage, aliquot into small volumes and store at –20˚C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.
Positive Control
- Human HEK293 cells, mouse 3T3-L1 cells and rat L6 cells.
Species Reactivity
H, M, R
Formulation
Purified immunoglobulin in phosphate buffered saline, pH 7.2, with 1% bovine serum albumin.
Short Description
4E-BP1
This product belongs to the following categories:
Related Products
References- Zhou, L., et al. (2005) 4E-binding protein phosphorylation and eukaryotic initiation factor-4E release are required for airway smooth muscle hypertrophy. Am. J. Respir. Cell Mol. Biol. 33(2):195-202.
- Wang, X., et al. (2005) Distinct signaling events downstream of mTOR cooperate to mediate the effects of amino acids and insulin on initiation factor 4E-binding proteins. Mol. Cell Biol. 25(7):2558-2572
- Li, W. and B.E. Sumpio (2005) Strain-induced vascular endothelial cell proliferation requires PI3K-dependent mTOR-4E-BP1 signal pathway. Am. J. Physiol. Heart Circ. Physiol. 288(4):H1591-H1597.
- Gingras, A.C., et al. (2001) Hierarchical phosphorylation of the translation inhibitor 4E-BP1. Genes Dev. 15(21):2852-2864.
Datasheet 0801-W
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