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XP-5100Bt


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REDI-Ladder™ Molecular Weight Marker


Antibody Production


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Primary Antibodies

4-1BB Receptor Antibody

Background

4-1BB Receptor Biotin Conjugated Antibody

Source

Produced from sera of goats pre-immunized with highly pure (>98%) recombinant human 4-1BB (Receptor).

Conjugate

Biotin

Clonality / Clone

This is a polyclonal antibody.

Host

4-1BB Receptor antibody was raised in goat.

Please use goat secondary antibodies.

Preparation

Lyophilized from sterile-filtered PBS solution.

Application

ELISA:

To detect h4-1BB (Receptor) by direct ELISA (using 100 μl/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 μg/ml. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant h4-1BB (Receptor).

Western Blot:

To detect h4-1BB (Receptor) by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant h4-1BB (Receptor) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Tested Application

E, WB

Storage

4-1BB Receptor lyophilized antibody is stable for at least 6 months when kept at -20˚C. The biotinylated antibody should be reconstituted to a concentration of 0.1 - 1.0 mg/ml with sterile PBS solution containing 0.1% BSA. This solution can be stored at 4˚C for at least one month without detectable loss of activity. Frozen aliquots of this solution are stable for at least 6 months when kept at -20˚C. Avoid more than one freeze-thaw cycle.

Species Reactivity

H

Short Description

Biotin - Anti-Human 4-1BB (Receptor)

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Datasheet 1001-W

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