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Recombinant Proteins
4-1BB Ligand Recombinant Protein
Background
4-1BBL, a member of the TNF superfamily, is expressed in B cells, dendritic cells, activated T cells and macrophages. 4-1BBL binds to its receptor 4-1BB, and provides a co-stimulatory signal for T cell activation and expansion. The human 4-1BBL gene codes for a 254 amino acid type II transmembrane containing a 28 amino acid cytoplasmic domain, a 21 amino acid transmembrane domain, and a 205 amino acid extracellular domain. The soluble form of 4-1BBL contains the TNF-like portion of the extracellular domain of 4-1BBL. Recombinant human 4-1BBL is a soluble 19.5 kDa protein consisting of 185 amino acid residues.
Additional Names
TNFSF9, CD137L
Location
MREGPELSPD DPAGLLDLRQ GMFAQLVAQN VLLIDGPLSW YSDPGLAGVS LTGGLSYKED TKELVVAKAG VYYVFFQLEL RRVVAGEGSG SVSLALHLQP LRSAAGAAAL ALTVDLPPAS SEARNSAFGF QGRLLHLSAG QRLGVHLHTE ARARHAWQLT QGATVLGLFR VTPEIPAGLP SPRSE
Source
E.coli
Reactivity
n/a
Preparation
Biological activity was determined by the dose - dependent stimulation of IL - 8 production by human PBMC.The ED50 for this effect is 5 - 10 ng/ml. Please Note: Results may vary with PBMC donors.
Tested Application
n/a
Storage
The lyophilized 4-1BB Ligand recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted 4-1BB Ligand is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing 4-1BB Ligand recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Species Reactivity
n/a
Purity
Greater than 98% by SDS-PAGE gel and HPLC analyses.
Endotoxin level is less than 0.1 ng per μg (1EU/μg).
Short Description
Recombinant Human 4-1BB Ligand
This product belongs to the following categories:
Datasheet 0904-W
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