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Slides
3T3/BALB Cell Slide
Background
BALB/3T3 clone A31 (embryo; fibroblast)
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Description
3T3/BALB Cell Slide
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Source
Fibroblast from 14- to 17-day-old BALB/c mouse embryos
Preparation
Mouse Balb/3T3 clone A31 cells were cultured in DMEM with 4 mM L-glutamine and harvested at the log phase of growth. The BALB/3T3 cells were fixed in 4% paraformaldehyde and arrayed on a 12-well (5 mm) adhesive coated slide, with each well’s surface specifically treated to enhance cellular attachment and to minimize background staining. Each well was loaded with approximately 7.5 x 103 BALB/3T3 cells. A follow-up heat dehydration process was done to ensure the attachment of BALB/3T3 cells and the stability of cellular proteins.
Application
BALB/3T3 cell slide is provided for immunocytochemical analysis of protein and protein-protein interaction. Procedures for immunohistochemistry can be directly applied.
Tested Application
ICC
Storage
BALB/3T3 cell slide can be stored for up to 6 months at 4°C under desiccate conditions.
Species Reactivity
n/a
Species
Mouse
Short Description
Fibroblast from 14- to 17-day-old BALB/c mouse embryos
This product belongs to the following categories:
Datasheet 0803-W
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