|
Slides
293 Cell Slide
Background
293 (kidney; transformed with adenovirus 5 DNA)
|
Description
293 Cell Slide
|
Source
Human (embryonal) kidney transformed by sheared human adenovirus 5 (Ad 5) DNA
Preparation
Human 293 cells were cultured in Minimum Essential Medium (Eagle) with 2 mM L-glutamine and harvested at the log phase of growth. The 293 cells were fixed in 4% paraformaldehyde and arrayed on a 12-well (5 mm) adhesive coated slide, with each well’s surface specifically treated to enhance cellular attachment and to minimize background staining. Each well was loaded with approximately 7.5 x 103 293 cells. A follow-up heat dehydration process was done to ensure the attachment of 293 cells and the stability of cellular proteins.
Application
293 cell slide is provided for immunocytochemical analysis of protein and protein-protein interaction. Procedures for immunohistochemistry can be directly applied.
Tested Application
ICC
Storage
293 cell slide can be stored for up to 6 months at 4°C under desiccate conditions.
Species Reactivity
n/a
Species
Human
Short Description
Human kidney transformed by sheared human adenovirus 5 (Ad 5) DNA
This product belongs to the following categories:
Datasheet 0803-W
|
|
