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Primary Antibodies
14-3-3 tau Antibody
Background
14-3-3 tau Antibody.
Additional Names
YWHAQ, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide, 14-3-3, 1C5, HS1, 14-3-3 protein T-cell, 14-3-3 protein tau, 14-3-3 protein theta, protein tau, tyrosine 3/tryptophan 5 -monooxygenase activation protein, theta polypeptide
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Description
Left: Western blot analysis of 14-3-3 tau in human hippocampus lysate (35μg protein in RIPA buffer) using 14-3-3 tau antibody (0.03μg/mL).
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Source
14-3-3 tau antibody was raised against a synthetic peptide of 14-3-3 tau.
Purification
14-3-3 tau antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
14-3-3 tau antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Peptide ELISA: Antibody detection limit dilution 1:4,000. Western Blot: Approx 28kDa band observed in human brain (hippocampus) lysates (calculated MW of 27.8kDa according to NP_006817.1). Recommended concentration: 0.03-0.1μg/ml.
Tested Application
E, WB
Buffer
14-3-3 tau antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Blocking Peptide
Cat. No. 45-175P - 14-3-3 tau Peptide
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Positive Control
- Cat. No. XBL-10110 - Human Hippocampus Tissue Lysate
Species Reactivity
H, M, R, D
Protein Accession Number
NP_006817.1
Short Description
14-3-3 tau / YWHAQ
This product belongs to the following categories:
Related Products
References- Howlett KF, Sakamoto K, Garnham A, Cameron-Smith D, Hargreaves M. Resistance exercise and insulin regulate AS160 and interaction with 14-3-3 in human skeletal muscle. Diabetes. 2007 Jun;56(6):1608-14. Epub 2007 Mar 16.
Datasheet 08-01W
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