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Primary Antibodies
14-3-3 sigma Antibody
Background
14-3-3 sigma Antibody.
Additional Names
SFN, stratifin, HGNC:10773, 14-3-3 sigma
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Description
Left: Western blot analysis of 14-3-3 sigma in human skin lysate (35μg protein in RIPA buffer) using 14-3-3 sigma antibody (0.1μg/mL).
Below: Immunohistochemistry staining of 14-3-3 in human skin using 14-3-3 antibody (2.5μg/mL).
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Other Product Images
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Source
14-3-3 sigma antibody was raised against a synthetic peptide of 14-3-3 sigma.
Purification
14-3-3 sigma antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
14-3-3 sigma antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx. 28kDa band observed in Human Skin lysates (calculated MW of 27.8kDa according to NP_006133.1). Recommended concentration: 0.1-0.3μg/ml. Immunohistochemistry: In paraffin embedded Human Skin shows strong nuclear staining in selected cells of the basal layer . Recommended concentration, 2-3μg/ml.
Tested Application
E, WB, IHC
Buffer
14-3-3 sigma antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Blocking Peptide
Cat. No. 45-174P - 14-3-3 sigma Peptide
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Positive Control
- Cat. No. 1376 - Human Skin Tissue Lysate
Species Reactivity
H, M, R, D
Protein Accession Number
NP_006133.1
Short Description
14-3-3 sigma / Stratifin
This product belongs to the following categories:
Related Products
References- Lodygin D, Hermeking H. The role of epigenetic inactivation of 14-3-3sigma in human cancer. Cell Res. 2005 Apr;15(4):237-46. Review.
Datasheet 08-01W
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