|
Primary Antibodies
14-3-3 Antibody
Background
14-3-3 proteins are a family of highly conserved proteins that appear to have multiple roles in cell signaling (Bridges and Moorhead, 2005). The proteins are abundantly expressed in the brain and have been detected in the cerebrospinal fluid of patients with different neurological disorders (Berg et al., 2003). 14-3-3 proteins bind protein ligands that are typically phosphorylated on serine or threonine residues and regulate the functions of these binding partners by a number of different mechanisms (Silhan et al., 2004; Dougherty and Morrison, 2004). The 14-3-3 proteins affect a diverse array of cellular processes including the cell cycle and transcription, signal transduction and intracellular trafficking.
|
Description
Left: Western blot of rat hippocampal (Hipp) lysate showing immunolabeling of the ~29k 14-3-3 protein.
|
Purification
Affinity purified
Clonality / Clone
This is a polyclonal antibody.
Host
14-3-3 Protein antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Peptide corresponding to amino acid residues from the C-terminal region of 14-3-3 protein, conjugated to keyhole limpet hemocyanin (KLH).
Application
The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, chicken, human, mouse, non-human primates, Xenopus and zebra fish based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
Tested Application
WB
Buffer
14-3-3 antibody is supplied as affinity purified polyclonal antibody, 100 μl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50% glycerol.
Storage
14-3-3 antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year.
Species Reactivity
H, M, R, D, ZF, B, C, X
Short Description
(CT) 14-3-3 Protein Pan-Specific
This product belongs to the following categories:
Related Products
References- Berg D, Holzmann C, Riess O (2003) 14-3-3 Proteins in the nervous system. Nat Rev Neurosci 4:752-762.
- Bridges D, Moorhead GB (2005) 14-3-3 Proteins: a number of functions for a numbered protein. Sci STKE 2005:re10.
- Dougherty MK, Morrison DK (2004) Unlocking the code of 14-3-3. J Cell Sci 117:1875-1884.
Datasheet 0803-W
|
|
