Datasheet

RICK Antibody
CATALOG NUMBER: 2075

Availability: In stock

$0.00
Product Price Options
$0.00
Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (95%)
TESTED APPLICATIONS:ELISA, ICC, IF, WB
APPLICATIONS:RICK antibody can be used for detection of RICK by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1201 - HeLa Cell Lysate
 2) Cat. No. 1225 - Ramos Cell Lysate
 3) Cat. No. 1287 - EL4 Cell Lysate
 4) Cat. No. 1202 - A431 Cell Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 59 kDa

Observed: 59 kDa
IMMUNOGEN:RICK antibody was raised against a peptide corresponding to 20 amino acids near the amino terminus of human RICK.

The immunogen is located within the first 50 amino acids of RICK.
HOST SPECIES:Rabbit

Properties

PURIFICATION:RICK Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:RICK Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:RICK antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:RICK Antibody: CCK, RICK, RIP2, CARD3, GIG30, CARDIAK, UNQ277/PRO314/PRO34092, CARD-containing interleukin-1 beta-converting enzyme-associated kinase, CARD-containing IL-1 beta ICE-kinase
ACCESSION NO.:O43353
PROTEIN GI NO.:20455217
OFFICIAL SYMBOL:RIPK2
GENE ID:8767

Background

BACKGROUND:RICK Antibody: Apoptosis is mediated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. DD-containing serine/threonine kinase RIP regulates Fas-induced apoptosis. A novel CARD-containing serine/threonine kinase was recently identified and designated RICK/RIP2/CARDIAK for RIP-like interacting CLARP kinase, receptor interacting protein-2, and CARD-containing ICE associated kinase, respectively. RICK contains an N-terminal kinase catalytic domain and a C-terminal CARD domain. Overexpression of RICK induced apoptosis and activation of NF-κB and JNK. RICK interacts with members of the TRAF family, CLARP and caspase-1. Thus, RICK represents a novel kinase that regulates TNF and Fas induced-apoptosis and that is involved in the generation of proinflammatory cytokine IL-1β. The messenger RNA of RICK is expressed in multiple human tissues.
REFERENCES: 1) Inohara N, del Peso L, Koseki T, et al. RICK, a novel protein kinase containing a caspase recruitment domain, interacts with CLARP and regulates CD95-mediated apoptosis. J. Biol. Chem. 1998; 273:12296-300.
2) McCarthy JV, Ni J, and Dixit VM. RIP2 is a novel NF-κB-activating and cell death-inducing kinase. J. Biol. Chem. 1998; 273:16968-75.
3) Thome M, Hofmann K, Burns K, et al. Identification of CARDIAK, a RIP-like kinase that associates with caspase-1. Curr. Biol. 1998; 8:885-8.

For Research Use Only