Datasheet

RAIDD Antibody
CATALOG NUMBER: 1115

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Mouse: (78%)
TESTED APPLICATIONS:ELISA, ICC, IF, WB
APPLICATIONS:RAIDD antibody can be used for detection of RAIDD by Western blot at 0.5 - 1 μg/mL. A 22 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1201 - HeLa Cell Lysate
 2) Cat. No. 1204 - K562 Cell Lysate
 3) Cat. No. 17-001 - HeLa Cell Slide
PREDICTED MOLECULAR WEIGHT:22 kDa
IMMUNOGEN:RAIDD antibody was raised against a 19 amino acid peptide near the center of human RAIDD.

The immunogen is located within amino acids 90 - 140 of RAIDD.
HOST SPECIES:Rabbit

Properties

PURIFICATION:RAIDD Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:RAIDD Antibody is supplied in PBS containing 0.02% sodium azide.
STORAGE CONDITIONS:RAIDD antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:RAIDD Antibody: MRT34, RAIDD, Death domain-containing protein CRADD, Caspase and RIP adapter with death domain
ACCESSION NO.:AAB42217
PROTEIN GI NO.:1785557
OFFICIAL SYMBOL:CRADD
GENE ID:8738

Background

BACKGROUND:RAIDD Antibody: Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain (DD)-containing receptors, TNFR1 and Fas. The death signals are transduced by a group of DD-containing adapter molecules. A novel cell death adapter was recently identified by two independent groups and designated RAIDD (RIP-associated ICH-1/CED-3-homologous protein with DD) and CRADD (caspase and RIP adapter with DD)1, RAIDD contains a DD and a CARD (for caspase recruitment domain) which interact with RIP and caspase, respectively, to transduce death signals1, 3. RAIDD is constitutively expressed in many tissues and mediates apoptosis caused by Fas and TNFR-1.
REFERENCES: 1) Duan H, Dixit VM. RAIDD is a new 'death' adaptor molecule. Nature 1997;385:86-89
2) Ahmad M, Srinivasula SM, Wang L, Talanian RV, Litwack G, Fernandes-Alnemri T, Alnemri ES. CRADD, a novel human apoptotic adaptor molecule for caspase-2, and FasL/tumor necrosis factor receptor-interacting protein RIP. Cancer Res 1997 57:615-619
3) Hofmann K, Bucher P, Tschopp J. The CARD domain: a new apoptotic signalling motif. Trends Biochem Sci 1997;22:155-156 (RD1299)

For Research Use Only