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Fax:     1-858-513-2692

abservices@prosci-inc.com

Rabbit Antibody Production

  Pkg. 1 Pkg. 2 Pkg. 3 Pkg. 4 Individual Services
  $700 $1,335 $1,835 $2,835  
Anti-Sera Schedule * * * * $700
* 8 week schedule
* 2 rabbits
* 2 pre-bleeds (5 ml per animal)
* 8 immunizations (4 per animal)
* 6 production bleeds (3 per animal)
         
Peptide Synthesis   * * * $20/residue
* Up to 15 residues for pkgs. 2-4
* Up to 30 residues for custom pkg.
* Allow 2 weeks
         
Conjugation   * * * $275
* To carrier protein (KLH)
* Allow 1 week
         
ELISA Report   * * * $60
* On 1st bleed for pkgs. 2-4          
Affinity Purification     *   $500
* 25 ml serum purified
* Includes reusable column
         
Large Scale Affinity Purification       * $1,500
* 150 ml serum purified
* Includes reusable column
         
Protein A or G Purification         $375
* 25 ml serum purified          
Custom Antibody Screening          
* Screening panel of ProSci IHC tissues         $300
* Screen 13 ProSci Westen Blot lysates         $300
Project Extension (per animal)         $120
* 28 Days
* 1 immunization
* 2 production bleeds (20-25 ml each)
         
Project Exsanguination (per animal)         $75
* Series of 2 bleeds for total of ~60 ml          


Example of a 2 rabbit custom antibody schedule:


Week Procedure Detailed Procedure Volume/Rabbit
0 Pre-bleed Pre-immune serum collection ~ 5 ml
0 Immunize 200 ug/rabbit in Complete Freund's Adjuvant (CFA)  
2 Immunize 100 ug/rabbit in Incomplete Freund's Adjuvant (IFA)  
4 Immunize 100 ug/rabbit in Incomplete Freund's Adjuvant (IFA)  
5 Bleed 1st production bleed 20-25 ml
6 Immunize 100 ug/rabbit in Incomplete Freund's Adjuvant (IFA)  
7 Bleed 2nd production bleed 20-25 ml
8 Bleed 3rd production bleed 20-25 ml

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