Datasheet

ORAI3 Antibody [1B4F1]
CATALOG NUMBER: PM-4913

Availability: In stock

$0.00
Product Price Options

* Required Fields

$0.00
Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Rat
TESTED APPLICATIONS:ELISA, WB
APPLICATIONS:ORAI3 antibody can be used for detection of ORAI3 by Western blot at 2 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1462 - Rat Lung Tissue Lysate
IMMUNOGEN:A 19 amino acid synthetic peptide from near the carboxy terminus of human ORAI3.
HOST SPECIES:Mouse

Properties

PURIFICATION:ORAI3 Monoclonal Antibody is immunoaffinity chromotography purified IgG.
PHYSICAL STATE:Liquid
BUFFER:ORAI3 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:ORAI3 monoclonal antibody can be stored at -20˚C, stable for one year.
CLONALITY:Monoclonal
ISOTYPE:IgG2b
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:ORAI3 Antibody [1B4F1] : TMEM142C
ACCESSION NO.:Q9BRQ5
PROTEIN GI NO.:74732916
OFFICIAL SYMBOL:ORAI3
GENE ID:93129

Background

BACKGROUND:ORAI3 Monoclonal Antibody: Antigen stimulation of immune cells triggers Ca++ entry t hrough Ca++ release-activated Ca++ (CRAC) channels. ORAI3 is one of two mammalian homologs to ORAI1, a recently identified four-transmembrane spanning protein that is an essential component of CRAC. All three homologs have been shown to function as Ca++ plasma membrane channels gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor. However, ORAI3 channels failed to produce detectable Ca++ selective currents in cells co-transfected with ORAI3 and STIM1, indicating that ORAI3 channels undergo a lesser degree of depotentiation than ORAI1 or ORAI2. Na+ currents through ORAI1, 2 and 3 channels were equally inhibited by extracellular Ca++, indicating that each have similar affinities for Ca++ within the selectivity filter. This antibody is predicted to have no cross-reactivity to ORAI1 or ORAI2. Larger molecular weight bands are sometimes seen in SDS-PAGE; these may represent post-translationally modified ORAI 3.
REFERENCES: 1) Lewis RS. Calcium signaling mechanisms in T lymphocytes. Annu. Rev. Immunol. 2001; 19:497-521.
2) Feske S, Gwack Y, Prakriya M, et al. A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function. Nature 2006; 441:179-85.
3) Soboloff J, Spassova MA, Dziadek MA, et al. Calcium signals mediated by STIM and Orai proteins - a new paradigm in inter-organelle communication. Biochim. Biophys. Acta. 2006; 1763:1161-8.
4) Mercer JC, DeHaven WI, Smyth JT, et al. Large store-operated calcium selective currents due to co-expression of Orai1 or Orai2 with the intracellular calcium sensor, Stim1. J. Biol. Chem. 2006; 281:24979-90.

For Research Use Only