Datasheet

NIK Antibody
CATALOG NUMBER: 1129

Availability: In stock

$0.00
Product Price Options
$0.00
Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Mouse: (94%)
TESTED APPLICATIONS:ELISA, WB
APPLICATIONS:NIK antibody can be used for detection of NIK by Western blot at 1 - 2 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1210 - HEK293 Cell Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 104 kDa
IMMUNOGEN:NIK antibody was raised against a 17 amino acid peptide near the carboxy terminus of human NIK.

The immunogen is located within the last 50 amino acids of NIK.
HOST SPECIES:Rabbit

Properties

PURIFICATION:NIK Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:NIK Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:NIK antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:NIK Antibody: HS, NIK, HSNIK, FTDCR1B, Mitogen-activated protein kinase kinase kinase 14, NF-kappa-beta-inducing kinase, HsNIK
ACCESSION NO.:Q99558
PROTEIN GI NO.:92090612
OFFICIAL SYMBOL:MAP3K14
GENE ID:9020

Background

BACKGROUND:NIK Antibody: Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFα, LPS and mitogens. A serine/threonine protein kinase which mediates NF-κB activation by IL-1, TNFα and CD95 was identified recently and designated NIK (for NF-κB inducing kinase). NIK is an activator of IκB kinase alpha and beta (IKKα and IKKβ). Therefore, NIK is a key molecule in the NF-κB signaling pathway leading to the induction of a variety of gene expression in response to proinflammatory cytokines and bacteria products.
REFERENCES: 1) Malinin NL, Boldin MP, Kovalenko AV, et al. MAP3K-related kinase involved in NF-κB induction by TNF, CD95 and IL-1. Nature 1997; 385:540-4.
2) Regnier CH, Song HY, Gao X, et al. Identification and characterization of an IκB kinase. Cell 1997; 90:373-83.
3) Woronicz JD, Gao X, Cao Z, et al. IκB kinase-β: NF-κB activation and complex formation with IκB kinase-α and NIK. Science 1997; 278:866-9.
4) Ling L, Cao Z, and Goeddel D. NF-κB-inducing kinase activates IKK-α by phosphorylation of Ser-176. Proc. Natl. Acad. Sci. USA 1998; 95:3792-7.

For Research Use Only