Datasheet

Neurturin Antibody
CATALOG NUMBER: 1121

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Mouse: (81%)
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:Neurturin antibody can be used for detection of neurturin by Western blot. An approximate 14 kDa band of the full length recombinant NTN was detected. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1205 - Jurkat Cell Lysate
 2) Cat. No. 1303 - Human Brain Tissue Lysate
 3) Cat. No. 10-301 - Human Brain Tissue Slide
PREDICTED MOLECULAR WEIGHT:14 kDa
IMMUNOGEN:Neurturin antibody was raised against a 16 amino acid peptide near the carboxy terminus of human Neurturin.

The immunogen is located within the last 50 amino acids of Neurturin.
HOST SPECIES:Rabbit

Properties

PURIFICATION:Neurturin Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:Neurturin Antibody is supplied in PBS containing 0.02% sodium azide.
STORAGE CONDITIONS:Neurturin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:Neurturin Antibody: NTN, Neurturin
ACCESSION NO.:NP_004549
PROTEIN GI NO.:4758826
OFFICIAL SYMBOL:NRTN
GENE ID:4902

Background

BACKGROUND:Neurturin Antibody: Glial cell line-derived neurotrophic factor (GDNF) plays key roles in the control of vertebrate neuron survival and differentiation. A novel neurotrophic factor was recently cloned from human and mouse and designated neurturin. Physiological responses to neurturin (NTN) require the presence of receptor tyrosine kinase RET and a novel glycosylphosphatidylinositol linked receptor NTNRalpha. The cDNAs encoding NTNRalpha from human, rat, chicken, and mouse have been cloned recently and termed GDNFRβ, Ret ligand 2 (RETL2) or TGF-beta-related neurotrophic factor receptor 2 (TrnR2) and nominated as GFRα-2 recently. NTN binds to and forms a complex with GFRα-2 and the Ret PTK and activates the RET receptor tyrosine kinase pathway. Both NTN and GDNF can activate the MAP kinase and phosphatidylinositol 3-kinase pathways and play a critical role in the development of many neuronal populations. Neurturin and GDNF define a new family of neurotrophic factors.
REFERENCES: 1) Kotzbauer PT, Lampe PA, Heuckeroth RO, et al. Neurturin, a relative glial-cell-line-derived neurotrophic factor. Nature 1996;384:467-470
2) Heuckeroth RO, Kotzbauer P, Copeland NG, et al. Neurturin, a novel neurotrophic factor, is localized to mouse chromosome 17 and human chromosome 19p13.3. Genomics 1997;44(1):137-40
3) Klein RD, Sherman D, Ho WH, et al. GPI-linked protein that interacts with Ret to form a candidate neurturin receptor. Nature 1997;387:717-721
4) Buj-Bello A, Adu J, Pinon LG, et al. Neurturin responsiveness requires a GPI-linked receptor and the Ret receptor tyrosine kinase. Nature 1997;387:721-724

For Research Use Only