Datasheet

Nephrin Antibody
CATALOG NUMBER: 5615

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:H
TESTED APPLICATIONS:E, IHC
APPLICATIONS:Nephrin antibody can be used for detection of Nephrin by immunohistochemistry at 5 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 10-401-Human Kidney Tissue Slide
IMMUNOGEN:Nephrin antibody was raised against a 16 amino acid synthetic peptide from near the center of human Nephrin.
HOST SPECIES:Rabbit

Properties

PURIFICATION:Nephrin Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:Nephrin Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:Nephrin antibody can be stored at 4˚C for three months and-20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:This is a polyclonal Nephrin Antibody.
ISOTYPE:IgG
CONJUGATE:None

Additional Info

ALTERNATE NAMES:Nephrin Antibody: CNF, NPHN, nephrin, Nephrin, Renal glomerulus-specific cell adhesion receptor
ACCESSION NO.:NP_004637
PROTEIN GI NO.:4758822

Background

BACKGROUND
Nephrin Antibody: Nephrin is strongly expressed in renal glomeruli and is a member of the immunoglobulin family of cell adhesion molecules. Mutations in the Nephrin gene result in congenital nephrotic syndrome, an autosomal-recessive disorder characterized by massive proteinuria in utero and nephrosis at birth. Renal glomeruli allow normal kidneys to filter plasma so that it is very pure. Nephrin is expressed in the podocyte slit-diaphragm of the renal glomeruli in a manner that suggests that Nephrin molecules homodimerize in an anti-parallel fashion similar to cadherin interactions in adherens junctions. Thus, Nephrin may constitute the entire extracellular structure of the slit-diaphragm.
REFERENCES
1) Kestila M, Lenkkeri U, Mannikko M, et al. Positionally cloned gene for a novel glomerular protein-Nephrin-is mutated in congenital nephrotic syndrome. Mol. Cell 1998; 1:575-582.
2) Tryggvason K. Unraveling the mechanisms of glomerular ultrafiltration: nephrin, a key component of the slit diaphragm. J. Am. Soc. Nephrol. 1999; 10:2440-5
3) Tryggvason K and Wartiovaara J. Molecular basis of glomerular permselectivity. Curr. Opin. Nephrol. Hypertens. 2001; 10:543-9.

For Research Use Only