Datasheet

MyD88 Antibody [2E9C2]
CATALOG NUMBER: PM-4673

Availability: In stock

$0.00
Product Price Options
$0.00
Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:MyD88 antibody can be used for detection of MyD88 by Western blot at 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1287 - EL4 Cell Lysate
 2) Cat. No. 95-104 - MyD88 Recombinant Protein
 3) Cat. No. 10-501 - Human Heart Tissue Slide
IMMUNOGEN:Mouse monoclonal MyD88 antibody was raised against a recombinant protein corresponding to amino acids 176 to 280 of human MyD88.
HOST SPECIES:Mouse

Properties

PURIFICATION:MyD88 Monoclonal Antibody is immunoaffinity chromotography purified IgG.
PHYSICAL STATE:Liquid
BUFFER:MyD88 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:MyD88 monoclonal antibody can be stored at -20˚C, stable for one year.
CLONALITY:Monoclonal
ISOTYPE:IgG2b
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:MyD88 Antibody [2E9C2] : MYD88D
ACCESSION NO.:AAC50954
PROTEIN GI NO.:1814020
OFFICIAL SYMBOL:MYD88
GENE ID:4615

Background

BACKGROUND:MyD88 Monoclonal Antibody: The pro-inflammatory cytokine IL-1 induced cellular response requires IL-1 receptor complex including IL-1RI and IL-1RAcP. MyD88 has been identified as an adapter molecule in the IL-1 signaling pathway. MyD88 associates with and recruits IRAK to the IL-1 receptor complex in response to IL-1 treatment and dominant negative form of MyD88 attenuates IL-1R-mediated NF-κB activation. MyD88 is also employed as a regulator molecule by IL-18 receptor and human Toll receptor, which are members in the Toll/IL-1R family of receptors. Targeted disruption of the MyD88 gene results in lose of cellular responses to IL-1 and IL-18, and MyD88-deficient mice lack responses to bacterial product LPS that employs Toll-like receptors 2 and 4 (TLR2 and TLR4) as the signaling receptors. MyD88 is a general adapter protein for the Toll/IL-1R family of receptors and plays an important role in the inflammatory response induced by cytokines IL-1 and IL-18 and endotoxin. MyD88 gene is expressed in many tissues.
REFERENCES: 1) Muzio M, Ni J, Feng P, et al. IRAK (Pelle) family member IRAK-2 and MyD88 as proximal mediators of IL-1 signaling. Science 1997; 278:1612-5.
2) Adachi O, Kawai T, Takeda K, et al. Targeted disruption of the MyD88 gene results in loss of IL-1- and IL-18-mediated function. Immunity 1998; 9:143-50.
3) Medzhitov R, Preston-Hurlburt P, Kopp E, et al. MyD88 is an adaptor protein in the hToll/IL-1 receptor family signaling pathways. Mol. Cell 1998; 2:253-8.
4) Kawai T, Adachi O, Ogawa T, et al. Unresponsiveness of MyD88-deficient mice to endotoxin. Immunity 1999; 11:115-22.

For Research Use Only