Datasheet

MYD88 Antibody
CATALOG NUMBER: 2125

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Pig: (100%), Sheep: (100%), Bovine: (100%), Chicken: (100%), Rat: (100%)
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:MYD88 antibody can be used for detection of MyD88 by Western blot 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1205 - Jurkat Cell Lysate
 2) Cat. No. 1201 - HeLa Cell Lysate
 3) Cat. No. 1301 - Human Heart Tissue Lysate
 4) Cat. No. 10-501 - Human Heart Tissue Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 35 kDa

Observed: 36 kDa
IMMUNOGEN:MD88 antibody was raised against a 16 amino acid peptide near the center of human MD88.

The immunogen is located within amino acids 220 - 270 of MYD88.
HOST SPECIES:Rabbit

Properties

PURIFICATION:MYD88 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:MYD88 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:MYD88 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:MYD88 Antibody: Myeloid differentiation primary response 88, MYD88D
ACCESSION NO.:U70451
PROTEIN GI NO.:1763090
OFFICIAL SYMBOL:MYD88
GENE ID:4615

Background

BACKGROUND:MYD88 Antibody: The pro-inflammatory cytokine IL-1 induced cellular response requires IL-1 receptor complex including IL-1RI and IL-1RAcP. Recently, MyD88 was identified as an adapter molecule in the IL-1 signaling pathway. MyD88 associates with and recruits IRAK to the IL-1 receptor complex in response to IL-1 treatment and dominant negative form of MyD88 attenuates IL-1R-mediated NF-κB activation. MyD88 is also employed as a regulator molecule by IL-18 receptor and human Toll receptor, which are members in the Toll/IL-1R family of receptors. Targeted disruption of the MyD88 gene results in lose of cellular responses to IL-1 and IL-18, and MyD88-deficient mice lack responses to bacterial product LPS that employs Toll-like receptors 2 and 4 (TLR2 and TLR4) as the signaling receptors. MyD88 is a general adapter protein for the Toll/IL-1R family of receptors and plays an important role in the inflammatory response induced by cytokines IL-1 and IL-18 and endotoxin. MyD88 gene is expressed in many tissues.
REFERENCES: 1) Muzio M, Ni J, Feng P, Dixit VM. IRAK (Pelle) family member IRAK-2 and MyD88 as proximal mediators of IL-1 signaling. Science 1997; 278:1612-5
2) Adachi O, Kawai T, Takeda K, Matsumoto M, Tsutsui H, Sakagami M, Nakanishi K, Akira S. Targeted disruption of the MyD88 gene results in loss of IL-1- and IL-18-mediated function. Immunity 1998; 9:143-50

For Research Use Only