Monoclonal Antibody Production

ProSci has the capability to produce monoclonal antibodies in vitro utilizing a unique cell culture method designed for greater efficiency; or in vivo method by producing ascites in mice.

Monoclonal Antibody Cell Culture:

Cell Culture Production of Monoclonal Antibodies

ProSci now offers in-vitro cell culture for custom monoclonal antibody production. Our methods have demonstrated a 5-25 fold increase in yields over conventional monoclonal antibody cell culture techniques. Serum and serum-free medium formulations are available. Production usually takes 3-4 weeks depending upon the cell line. Purification using protein G or A is also available.

ProSci will adapt the cell lines to our medium and scale them up for production. Please note that once the cell lines are adapted, they can be frozen and will be ready for future cell culture monoclonal antibody production without further adaptation. We offer volume sizes from 500ml to 10L.

Ascites Production:

ProSci can produce antibody from a Balb/c-derived cell line through the production of ascites fluid. This is done by injecting the cell line into the primed peritoneal cavity of Balb/c mice, and harvesting the fluid. We can do this from a cell line produced through our custom monoclonal antibody production programs, or from a cell line you send to us.

Our ascites production includes:

• Mycoplasma screening
• Cell line amplification
• Injection of cells into primed Balb/c mice
• 2 taps to obtain ascites fluid

If you have any questions regarding our custom monoclonal antibody production services please contact us at abservices@prosci-inc.com, or by calling toll-free 888.513.9525.