Datasheet

Lin28 Antibody [1G9H9]
CATALOG NUMBER: PM-6143

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Mouse: (87%), Chicken: (79%)
TESTED APPLICATIONS:ELISA, WB
APPLICATIONS:Lin28 antibody can be used for detection of Lin28 by Western blot at 0.5 - 1 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1207 - Raji Cell Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 23 kDa
SPECIFICITY:At least two isoforms of Lin28 are known to exist; this antibody will detect both. Lin28 antibody will not cross-react with Lin28 Homolog B.
IMMUNOGEN:Mouse monoclonal Lin28 antibody was raised against a 15 amino acid peptide near the carboxy terminus of human Lin28.

The immunogen is located within the last 50 amino acids of Lin28.
HOST SPECIES:Mouse

Properties

PURIFICATION:Lin28 Monoclonal Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:Lin28 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:Lin28 Monoclonal antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Monoclonal
ISOTYPE:IgG2b
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:Lin28 Antibody [1G9H9] : CSDD1, LIN28, LIN-28, ZCCHC1, lin-28A
ACCESSION NO.:NP_078950
PROTEIN GI NO.:13375938
OFFICIAL SYMBOL:LIN28A
GENE ID:79727

Background

BACKGROUND:Lin28 Monoclonal Antibody: Lin28 is a transcription factor that was first identified through its key role in the pathway of developmental timing in C. elegans. The role of Lin28 in development suggested that it might be useful in the creation of stem cells that might be beneficial in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing Lin28 in addition to the transcription factors POU5F1, Sox2, and NANOG in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing Lin28, Sox2, POU5F1 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.
REFERENCES: 1) Ambros V. A hierarchy of regulatory genes controls a larva-to-adult developmental switch in C. elegans. Cell 1989; 57:49-57.
2) Carpenter MK, Rosler E, and Rao MS. Characterization and differentiation of human embryonic stem cells. Cloning Stem Cells 2003; 5:79-88.
3) Tyu J, Vodyanik MA, Smuga-Otto K, et al. Induced pluripotent stem cell lines derived from human somatic cells. Science 2007; 318:1917-20
4) Okita K, Nakagawa M, Hyenjong H, et al. Generation of mouse induced pluripotent stem cells without viral vectors. Science 2008; 322:949-53.

For Research Use Only