Datasheet

IRAK Antibody [1F12C1]
CATALOG NUMBER: PM-5315

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse
TESTED APPLICATIONS:ELISA, IHC-P, WB
APPLICATIONS:RAK antibody can be used for Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 95-112 - IRAK Recombinant Protein
IMMUNOGEN:Recombinant protein corresponding to amino acids 618 - 712 of human IRAK.
HOST SPECIES:Mouse

Properties

PURIFICATION:IRAK Monoclonal Antibody is immunoaffinity chromotography purified IgG.
PHYSICAL STATE:Liquid
BUFFER:IRAK Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:IRAK monoclonal antibody can be stored at -20˚C, stable for one year.
CLONALITY:Monoclonal
ISOTYPE:IgG1
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:IRAK Antibody [1F12C1] : IRAK, pelle
ACCESSION NO.:P51617
PROTEIN GI NO.:8928535
OFFICIAL SYMBOL:IRAK1
GENE ID:3654

Background

BACKGROUND:IRAK Monoclonal Antibody: Nuclear factor kappa B (NF-kappaB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFalpha and LPS. A serine/threonine protein kinase associated with IL-1 receptor (IRAK) and its homologue mouse pelle-like protein kinase (mPLK) were identified recently. IRAK is associated with the IL-1 receptor subunits IL-1RI and IL-1RAcP after IL-1 binding and serves as a signaling molecule to mediate IL-1 response. IRAK mediates a signaling cascade leading to NF-κB activation by members in IL-1 family including IL-1 and a novel cytokine IL-18 (also termed IGIF).
REFERENCES: 1) Cao Z,; Henzel WJ, and Gao X. IRAK: a kinase associated with the interleukin-1 receptor. Science 1996; 271:1128-31.
2) Trofimova M, Sprenkle AB, Green M; et al. Developmental and tissue-specific expression of mouse pelle-like protein kinase. J. Bio. Chem. 1996; 271: 17609-12.
3) Jianing Huang, Xiong Gao, Shyun Li, et al. Recruitment of IRAK to the interleukin 1 receptor complex requires interleukin-1 receptor accessory protein. Proc. Natl. Acad. Sci. USA 1997; 94:12829-32.
4) Robinson D, Shibuya K, Mui A, et al. IGIF does not drive Th1 development but synergizes with IL-12 for interferon-gamma production and activates IRAK and NF-kB. Immunity 1997; 7:571-81.

For Research Use Only