Datasheet

IRAK Antibody
CATALOG NUMBER: 1007

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (85%)
TESTED APPLICATIONS:ELISA, ICC, IF, IP, WB
APPLICATIONS:IRAK antibody can be used for Western blot at 1 μg/mL and for immunoprecipitation with 2 to 4 μg per sample. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1201 - HeLa Cell Lysate
 2) Cat. No. 1208 - THP-1 Cell Lysate
 3) Cat. No. 17-001 - HeLa Cell Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 78 kDa

Observed: 80 kDa
SPECIFICITY:At least three isoforms of IRAK are known to exist; this antibody will detect all three isoforms. IRAK antibody is predicted to not cross-react with other members of the IRAK protein family.
IMMUNOGEN:IRAK antibody was raised against a peptide corresponding to 13 amino acids near the carboxy terminus of human IRAK.

The immunogen is located within the last 50 amino acids of IRAK.
HOST SPECIES:Rabbit

Properties

PURIFICATION:IRAK Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:IRAK Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:IRAK antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:IRAK Antibody: IRAK, pelle, IRAK, Interleukin-1 receptor-associated kinase 1, IRAK-1
ACCESSION NO.:P51617
PROTEIN GI NO.:8928535
OFFICIAL SYMBOL:IRAK1
GENE ID:3654

Background

BACKGROUND:IRAK Antibody: Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFα and LPS. A serine/threonine protein kinase associated with IL-1 receptor (IRAK) and its homologue mouse pelle-like protein kinase (mPLK) were identified recently. IRAK is associated with the IL-1 receptor subunits IL-1RI and IL-1RAcP after IL-1 binding and serves as a signaling molecule to mediate IL-1 response. IRAK mediates a signaling cascade leading to NF-κB activation by members in IL-1 family including IL-1 and a novel cytokine IL-18 (also termed IGIF).
REFERENCES: 1) Cao Z; Henzel WJ; Gao X. IRAK: a kinase associated with the interleukin-1 receptor. Science 1996;271:1128-31.
2) Trofimova M; Sprenkle AB; Green M; Sturgill TW; Goebl MG; Harrington MA. Developmental and tissue-specific expression of mouse pelle-like protein kinase. J Bio Chem 1996; 271: 17609-1
3) Jianing Huang, Xiong Gao, Shyun Li, and Zhaodan Cao. Recruitment of IRAK to the interleukin 1 receptor complex requires interleukin-1 receptor accessory protein. Proc Natl Acad Sci USA 1997;94:12829-12832
4) Robinson D, Shibuya K, Mui A, Zonin F, Murphy E, Sana T, Hartley SB, Menon S, Kastelein R, Bazan F, O'Garra A. IGIF does not drive Th1 development but synergizes with IL-12 for interferon-γ production and activates IRAK and NF-κB. Immunity 1997;7:571-581

For Research Use Only