Datasheet

IRAK-2 Antibody
CATALOG NUMBER: 2123

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Mouse: (77%)
TESTED APPLICATIONS:ELISA, ICC, IF, WB
APPLICATIONS:IRAK-2 antibody can be used for detection of IRAK2 by Western blot at 2 μg/mL. A 65 kDa band should be detected. This polyclonal antibody can also detect IRAK2 by immunocytochemistry at 2 μg/mL. For immunofluorescence start at 10 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1201 - HeLa Cell Lysate
 2) Cat. No. 1204 - K562 Cell Lysate
 3) Cat. No. 17-001 - HeLa Cell Slide
PREDICTED MOLECULAR WEIGHT:65 kDa
SPECIFICITY:Anti-IRAK2 has no cross response to IRAK.
IMMUNOGEN:IRAK2 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human IRAK2.

The immunogen is located within amino acids 530 - 580 of IRAK-2.
HOST SPECIES:Rabbit

Properties

PURIFICATION:IRAK-2 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:IRAK-2 Antibody is supplied in PBS containing 0.02% sodium azide.
STORAGE CONDITIONS:IRAK-2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:IRAK-2 Antibody: IRAK-2, Interleukin-1 receptor-associated kinase-like 2, IRAK-2
ACCESSION NO.:AF026273
PROTEIN GI NO.:2653876
OFFICIAL SYMBOL:IRAK2
GENE ID:3656

Background

BACKGROUND:IRAK-2 Antibody: The pro-inflammatory cytokine IL-1 induces cellular response through two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1 receptor-associated kinase (IRAK) mediates activation of NF-κB, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family was recently identified and designated IRAK2. Both IRAK and IRAK2 recruit to the subunits of the IL-1R complex after IL-1 binding and lead to NF-κB activation. IRAKs also associate with Toll like receptor (TLR) and the dominant negative mutants of IRAKs inhibit LPS-induced NF-κB activation. Members in IRAK/Pelle family play a central role in IL-1R and TLR mediated inflammatory response. IRAK2 is expressed in a variety of human tissues.
REFERENCES: 1) Muzio M, Ni J, Feng P, Dixit VM. IRAK (Pelle) family member IRAK-2 and MyD88 as proximal mediators of IL-1 signaling. Science 1997;278:1612-5.
2) Zhang FX, Kirschning CJ, Mancinelli R, Xu XP, Jin Y, Faure E, Mantovani A, Rothe M, Muzio M, Arditi M. Bacterial lipopolysaccharide activates nuclear factor-κB through interleukin-1 signaling mediators in cultured human dermal endothelial cells and mononuclear phagocytes. J Biol Chem 1999;274:7611-4.
3) Yang RB, Mark MR, Gurney AL, Godowski PJ. Signaling events induced by lipopolysaccharide-activated toll-like receptor 2. J Immunol 1999;163:639-43. (RD1299)

For Research Use Only