Datasheet

IL-33 Antibody [12H6D12]
CATALOG NUMBER: PM-5041

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse
TESTED APPLICATIONS:ELISA, IHC-P, WB
APPLICATIONS:IL-33 antibody can be used for the detection of IL-33 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 95-106 - IL-33 Recombinant Protein
 2) Cat. No. 11-521 - Human Lymphoid Tissue Tissue Slide
IMMUNOGEN:Mouse monoclonal IL-33 antibody was raised against the recombinant IL-33 protein.
HOST SPECIES:Mouse

Properties

PURIFICATION:IL-33 Monoclonal Antibody is immunoaffinity chromotography purified IgG.
PHYSICAL STATE:Liquid
BUFFER:IL-33 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:IL-33 monoclonal antibody can be stored at -20˚C, stable for one year.
CLONALITY:Monoclonal
ISOTYPE:IgG1
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:IL-33 Antibody [12H6D12] : DVS27, IL1F11, NF-HEV, NFEHEV, C9orf26
ACCESSION NO.:NP_254274
PROTEIN GI NO.:15559209
OFFICIAL SYMBOL:IL33
GENE ID:90865

Background

BACKGROUND:IL-33 Monoclonal Antibody: Interleukin-33 (IL-33) is a recently identified member of the IL-1 family of cytokines whose other members include IL-1αβ, IL-1Ra and IL-18. Its receptor has been shown to be ST2, an IL-1 receptor family member that also acts as a negative regulator of TLR-IL-1R signaling and IL-1R accessory protein (IL-1RAcP). Receptor binding of IL-33 activates NF-κB and MAP kinases and induces the expression of TH2-associated cytokines such as IL-4, IL-5 and IL-6. Prolonged IL-33 treatment of mice led to the development of eosinophilia, splenomegaly, and severe pathological changes in mucosal organs such as lungs, esophagus and small intestine. Recent experiments have shown that IL-33 can also co-localize with heterochromatin and possesses transcriptional repressor activities, indicating that IL-33 may function as both a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties. Despite its predicted molecular weight, IL-33 will often run at higher molecular weight in SDS-PAGE.
REFERENCES: 1) Schmitz J, Owyang A, Oldham E, et al. IL-33, and interleukin-1-like cytokine that signals via the IL-1 receptor-related protein ST2 and induces T helper type 2-associated cytokines. Immunity 2005; 23:479-90.
2) Dinarello CA. Interleukin-18, a proinflammatory cytokine. Eur. Cytokine Netw. 2000; 11:483-6.
3) Brint EK, Xu D, Liu H, et al. ST2 is an inhibitor of interleukin 1 receptor and Toll-like receptor 4 signaling and maintains endotoxin tolerance. Nat. Immunol. 2004; 5:373-9.
4) Chackerian AA, Oldham ER, Murphy EE, et al. IL-1 receptor accessory protein and ST2 comprise the IL-33 receptor complex. J. Immunol. 2007; 179:2551-5.

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