Datasheet

ICAD Antibody
CATALOG NUMBER: 2003

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Mouse
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:ICAD antibody can be used for detection of of ICAD by Western blot at 1:1000 dilution. A 45 kDa band can be detected. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1402 - Mouse Lung Tissue Lysate
PREDICTED MOLECULAR WEIGHT:45 kDa
IMMUNOGEN:ICAD antibody was raised against a 20 amino acid peptide near the carboxy terminus of mouse ICAD.

The immunogen is located within the last 50 amino acids of ICAD.
HOST SPECIES:Rabbit

Properties

PURIFICATION:ICAD Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:ICAD Antibody is supplied in PBS containing 0.02% sodium azide.
STORAGE CONDITIONS:ICAD antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:ICAD Antibody: ICAD, DFF35, Dff45, ICAD-L, ICAD-S, A330085O09Rik, Icad, DNA fragmentation factor subunit alpha, DNA fragmentation factor 45 kDa subunit, DFF-45
ACCESSION NO.:O54786
PROTEIN GI NO.:9087146
OFFICIAL SYMBOL:Dffa
GENE ID:13347

Background

BACKGROUND:ICAD Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human DNA fragmentation factor (DFF) was identified recently which was cleaved by caspase-3 during apoptosis. Mouse homologue of human DFF was identified as a DNase inhibitor designated ICAD, for inhibitor of caspase-activated DNase. Upon cleavage of DFF/ICAD, a caspase activated deoxyribonuclease (CAD) is released and activated and eventually causes the degradation of DNA in the nuclei. Therefore, the cleavage of CAD inhibitor molecule DFF/ICAD, which causes DNase activation and DNA degradation, is the hallmark of apoptotic cell death.
REFERENCES: 1) Liu X, Zou H, Slaughter C, Wang X. DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis. Cell 1997;89:175-184
2) Enari M, Sakahira H, Yokoyama H, Okawa K, Iwamatsu A, Nagata S. A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD. Nature 1998;391:43-50
3) Sakahira H, Enari M, Nagata S. Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis. Nature 1998;391:96-99
4) Wyllie A. Apoptosis. An endonuclease at last. Nature 1998;39120-21 (RD1299)

For Research Use Only