Datasheet

ERGIC53 Monoclonal Antibody
CATALOG NUMBER: PM-7213

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse
TESTED APPLICATIONS:ELISA, ICC, WB
APPLICATIONS:ERGIC53 antibody can be used for detection of ERGIC53 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1209 - HL60 Cell Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 56 kDa
SPECIFICITY:At least two isoforms of ERGIC53 are known to exist.
IMMUNOGEN:Mouse monoclonal ERGIC53 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human ERGIC53.
HOST SPECIES:Rabbit

Properties

PURIFICATION:ERGIC53 Monoclonal Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:ERGIC53 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:ERGIC53 Monoclonal antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Monoclonal
ISOTYPE:IgG1
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:ERGIC53 Monoclonal Antibody: Lectin mannose-binding 1, LMAN1, endoplasmic reticulum-golgi intermediate compartment protein 53, ERGIC-53, F5F8D, FMFD1, MCFD1, MR60, gp58
ACCESSION NO.:NP_005561
PROTEIN GI NO.:5031873
OFFICIAL SYMBOL:LMAN1
GENE ID:3998

Background

BACKGROUND:ERGIC53 Monoclonal Antibody: ERGIC53, also known as lectin mannose-binding 1 (LMAN1) protein is a type I integral membrane protein localized in the intermediate region between the endoplasmic reticulum and the Golgi, presumably recycling between the two compartments. The protein is a mannose-specific lectin and is a member of a novel family of plant lectin homologs in the secretory pathway of animal cells. Mutations in the gene are associated with a coagulation defect. Using positional cloning, the gene was identified as the disease gene leading to combined factor V-factor VIII deficiency, a rare, autosomal recessive disorder in which both coagulation factors V and VIII are diminished.
REFERENCES: 1) Schindler R, Itin C, Zerial M, et al. ERGIC-53, a membrane protein of the ER-Golgi intermediate compartment, carries an ER retention motif. Eur. J. Cell Biol. 1993; 61:1-9.
2) ERGIC-53 is a functional mannose-selective and calcium-dependent human homologue of leguminous lectins. Mol. Biol Cell 1996; 7:483-93
3) Zhang YC, Zhou Y, Yang CZ, et al. A review of ERGIC-53: its structure, functions, regulation and relations with diseases. Histol. Histopathol. 2009; 24:1193-204.

For Research Use Only