Datasheet

Eotaxin Antibody
CATALOG NUMBER: 1123

Availability: In stock

$0.00
Product Price Options

* Required Fields

$0.00
Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Pig: (80%)
TESTED APPLICATIONS:ELISA, ICC, IF, WB
APPLICATIONS:Eotaxin antibody can be used for detection of eotaxin by Western blot at 1 μg/mL. The recombinant protein can be used as positive control and monomer and homodimer are detected at approximate 9 and 18 kDa. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 10 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1201 - HeLa Cell Lysate
 2) Cat. No. 17-010 - HEK293 Cell Slide
PREDICTED MOLECULAR WEIGHT:9 - 18 kDa
IMMUNOGEN:Eotaxin antibody was raised in rabbits against a peptide corresponding to amino acids near the carboxy terminus of human eotaxin.

The immunogen is located within the last 50 amino acids of Eotaxin.
HOST SPECIES:Rabbit

Properties

PURIFICATION:Eotaxin Antibody is Antibody is DEAE purified.
PHYSICAL STATE:Liquid
BUFFER:Eotaxin Antibody is supplied in PBS containing 0.02% sodium azide.
STORAGE CONDITIONS:Eotaxin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:Eotaxin Antibody: SCYA11, SCYA11, Eotaxin, C-C motif chemokine 11
ACCESSION NO.:CAB07027
PROTEIN GI NO.:2462478
OFFICIAL SYMBOL:CCL11
GENE ID:6356

Background

BACKGROUND:Eotaxin Antibody: Chemokines play a key role in inflammation. The CC chemokine eotaxin is a potent and specific eosinophil chemoattractant that is expressed by a variety of cell types in certain inflammatory conditions. Some G-protein coupled chemokine receptors are also utilized as virus coreceptors for fusion and infection of cells. The eotaxin receptor CCR3 is required for HIV-1 entry into target cells such as microglia and eotaxin inhibits the infection of HIV-1.
REFERENCES: 1) Kitaura M, Nakajima T, Imai T, et al. Molecular cloning of human eotaxin, an eosinophil-selective CC chemokine, and identification of a specific eosinophil eotaxin receptor, CC chemokine receptor 3. J Biol Chem 1996;271:7725-30
2) Ponath PD, Qin S, Ringler DJ, et al. Cloning of the human eosinophil chemoattractant, eotaxin. Expression, receptor binding, and functional properties suggest a mechanism for the selective recruitment of eosinophils. J Clin Invest 1996;97:604-12
3) He J, Chen Y, Farzan M, et al. CCR3 and CCR5 are co-receptors for HIV-1 infection of microglia. Nature. 1997;385(6617):645-9
4) Choe H, Farzan M, Sun Y, et al. The β-chemokine receptors CCR3 and CCR5 facilitate infection by primary HIV-1 isolates. Cell. 1996;85:1135-48 (WD0500)

For Research Use Only