Datasheet

DOK1 Antibody
CATALOG NUMBER: 1107

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human
TESTED APPLICATIONS:ELISA, ICC, IF, WB
APPLICATIONS:DOK1 antibody can be used for detection of DOK1 expression by Western blot at 1 μg/mL. A 62 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1205 - Jurkat Cell Lysate
 2) Cat. No. 1208 - THP-1 Cell Lysate
 3) Cat. No. 1204 - K562 Cell Lysate
 4) Cat. No. 17-004 - K-562 Cell Slide
PREDICTED MOLECULAR WEIGHT:62 kDa
IMMUNOGEN:DOK1 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human DOK1.

The immunogen is located within amino acids 410 - 460 of DOK1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:DOK1 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:DOK1 Antibody is supplied in PBS containing 0.02% sodium azide.
STORAGE CONDITIONS:DOK1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:DOK1 Antibody: TP1, TLP1, p240, TROVE1, VAULT2, Docking protein 1, Downstream of tyrosine kinase 1
ACCESSION NO.:AAC51127
PROTEIN GI NO.:1848277
OFFICIAL SYMBOL:TEP1
GENE ID:7011

Background

BACKGROUND:DOK1 Antibody: Signals from most growth factors and cytokines are transduced by receptor tyrosine kinases or non-receptor tyrosine kinases. Activated tyrosine kinases phosphorylate their substrates, which mediate the cellular response to extracellular stimuli. A long-sought major substrate termed p62dok (downstream of tyrosine kinase) for many tyrosine kinases including c-kit, v-abl, v-Fps, v-Src, v-Fms, and activated EGF, PDGF, IGF, VEGF and insulin receptors was identified recently from human and mouse by several laboratories. Upon phosphorylation, p62dok forms a complex with the ras GTPase-activating protein (RasGAP). p62dok represents a new family with very recently identified p56dok.
REFERENCES: 1) Carpino N, Wisniewski D, Strife A, Marshak D, Kobayashi R, Stillman B, Clarkson B p62(dok): a constitutively tyrosine-phosphorylated, GAP-associated protein in chronic myelogenous leukemia progenitor cells. Cell 1997;88:197-204.
2) Yamanashi Y, Baltimore D Identification of the Abl- and rasGAP-associated 62 KDa protein as a docking protein, Dok. Cell 1997;88:205-211.
3) Holland SJ, Gale NW, Gish GD, Roth RA, Songyang Z, Cantley LC, Henkemeyer M, Yancopoulos GD, Pawson T. Juxtamembrane tyrosine residues couple the Eph family receptor EphB2/Nuk to specific SH2 domain proteins in neuronal cells. EMBO J 1997;16:3877-3888.
4) Di Cristofano A, Carpino N, Dunant N, Friedland G, Kobayashi R, Strife A, Wisniewski D, Clarkson B, Pandolfi PP, Resh MD. Molecular cloning and characterization of p56(dok-2) defines a new family of RasGAP-binding proteins. J Biol Chem 1998;273:4827-4830.

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