Datasheet

Daxx Antibody
CATALOG NUMBER: 1163

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Rat: (100%)
TESTED APPLICATIONS:ELISA, ICC, WB
APPLICATIONS:Daxx antibody can be used for detection of Daxx by Western blot at 1 μg/mL. A 120 kDa major band can be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1201 - HeLa Cell Lysate
 2) Cat. No. 17-001 - HeLa Cell Slide
PREDICTED MOLECULAR WEIGHT:120 kDa
IMMUNOGEN:Daxx antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human Daxx.

The immunogen is located within the last 50 amino acids of Daxx.
HOST SPECIES:Rabbit

Properties

PURIFICATION:Daxx Antibody is Antibody is DEAE purified.
PHYSICAL STATE:Liquid
BUFFER:Daxx Antibody is supplied in PBS containing 0.02% sodium azide.
STORAGE CONDITIONS:Daxx antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:Daxx Antibody: DAP6, EAP1, BING2, DAP6, Death domain-associated protein 6, Daxx, hDaxx
ACCESSION NO.:CAG33366
PROTEIN GI NO.:48146287
OFFICIAL SYMBOL:DAXX
GENE ID:1616

Background

BACKGROUND:Daxx Antibody: Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 and Fas. Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the ICE/CED-3 protease family. A novel DD-containing molecule was recently cloned from mouse, human and monkey and designated Daxx. Daxx binds specifically to the Fas death domain and enhances Fas induced apoptosis and activates the Jun N-terminal kinase (JNK) pathway. Daxx is widely expressed in fetal and adult human and mouse tissues indicating its important function in Fas signaling pathways.
REFERENCES: 1) Yang X, Khosravi-Far R, Chang HY, Baltimore D. Daxx, a novel Fas-binding protein that activates JNK and apoptosis. Cell 1997;89:1067-1076
2) Kiriakidou M, Driscoll DA, Lopez-Guisa JM, Strauss JF 3rd. Cloning and expression of primate Daxx cDNAs and mapping of the human gene to chromosome 6p21.3 in the MHC region. DNA Cell Biol 1997;16:1289-1298 (RD1299)

For Research Use Only