Datasheet

CXCR4 Antibody
CATALOG NUMBER: 1009

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (86%), Pig: (71%), Rat: (71%)
TESTED APPLICATIONS:ELISA, Flow, ICC, IF, IP, WB
APPLICATIONS:CXCR4 antibody can be used for Western blot at 1 - 2 μg/mL dilution and for immunoprecipitation and immunocytochemistry at 10 μg/mL. Immunofluorescence starting at 20 μg/mL. Flow Cytometry at 0.1 μg/ml
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1201 - HeLa Cell Lysate
 2) Cat. No. 17-001 - HeLa Cell Slide
SPECIFICITY:CXCR4 Antibody is predicted to not cross-react with other CXCR familiy members.
IMMUNOGEN:CXCR4 antibody was raised against a peptide corresponding to 14 amino acids near the amino terminus of human CXCR4.

The immunogen is located within the first 50 amino acids of CXCR4.
HOST SPECIES:Rabbit

Properties

PURIFICATION:CXCR4 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:CXCR4 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:CXCR4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:CXCR4 Antibody: FB22, HM89, LAP3, LCR1, NPYR, WHIM, CD184, LESTR, NPY3R, NPYRL, HSY3RR, NPYY3R, D2S201E
ACCESSION NO.:NP_003458
PROTEIN GI NO.:4503175
OFFICIAL SYMBOL:CXCR4
GENE ID:7852

Background

BACKGROUND:CXCR4 Antibody: Human immunodeficiency virus (HIV) and related viruses require coreceptors, in addition to CD4, to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors. Among them, CXCR4 (fusin, LESTR or HUMSTR) is a principal coreceptor for T-cell tropic strains of HIV-1 fusion and entry of human white blood cells. CXCR4 is also required for the infection by dual-tropic strains of HIV-1 and mediates CD-4 independent infection by HIV-2. The a-chemokine SDF-1 is the ligand for CXCR4 and prevents infection by T-tropic HIV-1. CXCR4 associates with the surface CD4-gp120 complex before HIV enters target cells. CXCR4 messenger RNA levels correlated with HIV-1 permissiveness in diverse human cell types. Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells. The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV binding sites.
REFERENCES: 1) Dimitrov DS. Cell 1997;91:721-730
2) Feng Y et al. Science 1996;272:872-7
3) Berson JF et al. J Virol 1996;70:6288-95
4) Doranz BJ et al. Cell 1996;85:1149-1158

For Research Use Only