Datasheet

CX3CR1 Antibody
CATALOG NUMBER: 2093

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (82%)
TESTED APPLICATIONS:ELISA, Flow, IF, IHC-P, WB
APPLICATIONS:CX3CR1 antibody can be used for detection of CX3CR1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL. Flow cytometry at 0.1 μg/ml.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1306 - Human Spleen Tissue Lysate
 2) Cat. No. 10-501 - Human Heart Tissue Slide
 3) Cat. No. 1466 - Rat Spleen Tissue Lysate
 4) Cat. No. 1405 – Mouse Kidney Tissue Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 50 kDa

Observed: 52 kDa
IMMUNOGEN:CX3CR1 antibody was raised against a 20 amino acid peptide near the amino terminus of human CX3CR1.

The immunogen is located within the first 50 amino acids of CX3CR1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:CX3CR1 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:CX3CR1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:CX3CR1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:CX3CR1 Antibody: V28, CCRL1, GPR13, CMKDR1, GPRV28, CMKBRL1, CX3C chemokine receptor 1, Beta chemokine receptor-like 1, C-X3-C CKR-1
ACCESSION NO.:NP_001328
PROTEIN GI NO.:4503171
OFFICIAL SYMBOL:CX3CR1
GENE ID:1524

Background

BACKGROUND:CX3CR1 Antibody: CX3CR1 is one of the chemokine receptors that are required as coreceptors for HIV infection. The genes encoding human, murine, and rat CX3CR1 were cloned and designated V28 and CMKBRL1, CX3CR1, and RBS11, respectively. The encoded seven transmembrane protein was recently identified as the receptor for a novel transmembrane molecule, fractalkine, and renamed CX3CR1. Recently, CX3CR1 was found to serve as a coreceptor for HIV-1 and HIV-2 envelope fusion and virus infection, which can be inhibited by fractokine. CX3CR1 mediates leukocyte migration and adhesion. CX3CR1 is expressed in a variety of human tissues and cell lines.
REFERENCES: 1) Raport CJ, Schweickart VL, Eddy RL JR, Shows TB, Gray PW. The orphan G-protein-coupled receptor-encoding gene V28 is closely related to genes for chemokine receptors and is expressed in lymphoid and neural tissues. Gene 1995;163:295-9
2) Combadiere C, Ahuja SK, Murphy PM. Cloning, chromosomal localization, and RNA expression of a human β chemokine receptor-like gene. DNA Cell Biol 1995;14:673-80
3) Combadiere C, Gao J, Tiffany HL, Murphy PM. Gene cloning, RNA distribution, and functional expression of mCX3CR1, a mouse chemotactic receptor for the CX3C chemokine fractalkine. Biochem Biophys Res Commun 1998;253:728-32
4) Harrison JK, Barber CM, Lynch KR. cDNA cloning of a G-protein-coupled receptor expressed in rat spinal cord and brain related to chemokine receptors. Neurosci Lett 1994;169:85-9

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