Custom Polyclonal Antibody Packages

We offer packages for client-provided antigens, single-peptide projects, peptide cocktail projects, and phospho-peptide projects.

Custom Antibody Package 1 uses a client-provided antigen, and includes an 8-week immunization protocol in a species of your choice. We can start a project with as little as 0.25mg of your antigen, or perform a complete immunization protocol with as little as 0.5mg of antigen in two chickens. We can perform additional services (ELISAs, purifications, western blotting, etc.) for any project to assist you in evaluating the antibodies produced.

Custom Antibody Package 2 includes complimentary antigen design, synthesis of a 15-residue peptide at >70% HPLC purity, conjugation of the peptide to a carrier protein, an 8-week immunization protocol in a species of your choice, and an ELISA to verify titers on the first production bleed; a titer of >1:5,000 is guaranteed if you use a peptide sequence we approved.

Custom Antibody Package 3 includes complimentary antigen design, synthesis of a 15-residue peptide at >70% HPLC purity, conjugation of the peptide to a carrier protein, an 8-week immunization protocol in a species of your choice, an ELISA to verify titers on the first production bleed, construction of an immuno-affinity column using project-peptide, and purification of 25mL of serum or yolk; a titer of >1:5,000 is guaranteed if you use a peptide sequence we approved.

Custom Antibody Package 4 includes complimentary antigen design, synthesis of a 15-residue peptide at >70% HPLC purity, conjugation of the peptide to a carrier protein, an 8-week immunization protocol in a species of your choice, an ELISA to verify titers on the first production bleed, construction of an immuno-affinity column using project peptide, and purification of 200mL of serum or yolk; a titer of >1:5,000 is guaranteed if you use a peptide sequence we approved.

Peptide Cocktail Antibody Production Packages include all of the services in Package 2 and Package 3 respectively, but include the synthesis, conjugation, and immunization of your choice of 2 or 3 peptides into one set of animals. This is an option that increases the likelihood of a peptide-based antibody working for multiple applications, as it doubles or triples the available number of epitopes for the animals’ immune systems to respond against. It also helps to reduce overall cost, as it minimizes the number of animals needed for a particular project.

Phospho-Specific Antibody Production Packages include all of the services in Package 2, 3, and 4 respectively, but include the synthesis, conjugation, and immunization of phospho peptides into a set of rabbits. Packages 2PN, 3PN, and 4PN also offer the synthesis of the corresponding non-phospho peptide sequence, used for isolating both the phospho and non-phospho antibodies for your target protein.

Additional services ProSci offers include:

• Peptide synthesis
• HPLC purification of peptide to >90% purity
• Conjugation of peptides and proteins to carrier proteins
• ELISA titration
• Protein A/G/L purification
• IgY purification
• Immuno-affinity purification
• Western blot analysis
• Immunohistochemistry analysis
• Project protocol extension
• Project exsanguination

Downloads:

Rabbit Info Sheet (PDF) Rabbit Order Form (PDF)

Chicken Info Sheet (PDF) Chicken Order Form (PDF)

Goat Info Sheet (PDF) Goat Order Form (PDF)

Background Information on Polyclonal Antibody Production:

• Animal Procedures
• Rabbit Care Information
• Chicken Care Information
• Goat Care Information

If you have any questions regarding our custom polyclonal antibody production packages, please contact us at abservices@prosci-inc.com, or by calling toll-free 888.513.9525.