Antibody Project Consulting


Determining which custom antibody service is right for your application is the first step towards initiating project. There are many factors that should be taken into account, including desired antibody quantity, desired antibody applications, and antibody specificity.

Each project type has its own set of advantages and disadvantages. One factor is to determine whether to go with a polyclonal, monoclonal, or single domain antibody. Another factor is the host animal should be used for development of the antibodies. The charts below offer the advantages and disadvantages for both factors.

Advantages & Disadvantages: Projects

 ProsConsSpecies
  • Unlimited quantity and availability of antibody
  • Lot-to-lot consistency
  • High specificity
  • Quick reproduction of antibody once hybridoma is developed
  • Longer production time
  • Requires a minimum of 4 mgs immunogen
  • Higher cost of development
  • Antibodies less likely to work for multiple applications
  • More difficult if using peptide immunogens
  • Low affinity
  • Quick turnaround
  • Inexpensive
  • High affinity
  • One antibody can work for a variety of applications
  • Requires less than 1mg immunogen
  • Multiple host species, including non-mammalian hosts
  • Quantity available limited by the number of bleeds
  • Lot-to-lot variability in characterization
  • Difficulty to reproduce
  • Chicken
  • Goat
  • Rabbit
  • Guinea Pig
  • Llama
  • Mouse
  • Rat
  • Bovine
  • Sheep
  • Small size, ~15kDa, for enhanced tissue penetration
  • Higher neutralization potential
  • High affinity and specificity
  • Lot-to-lot consistency
  • Requires less than 1mg antigen
  • Stable under extreme temperature and pH
  • Quick reproduction of antibody after development
  • Cost-effectuve large scale production
  • High development cost
  • Limited success binding small antigens (peptides & small molecules)
  • Llama

Advantages & Disadvantages: Species

 ProsConsIdeal Package
Chicken
Chicken
  • Non-mammalian host species
  • Good alternative for highly conserved mammalian proteins
  • Non-invasive (antibodies are purified from egg yolks)
  • Difficult to isolate antibodies from yolks
  • Highly conserved mammalian protein antigens
  • Not ideal for peptide projects
Polyclonal
Goat
Goat
  • Large mammalian host species
  • High quantity (250mL) bleeds for large lots
  • Serum can be used directly in initial assays
  • Not ideal for modified peptide
  • Largen antigen quantities
Large Volume Polyclonal
Guinea Pig
Guinea Pig
  • More blood provided than a rat
  • Serum can be used directly in initial assay
  • Smaller serum volumes
Medium Volume Polyclonal
Llama
Llama
  • Large mammalian host species
  • Heavy-chain only antibodies 
  • Serum can be used directly
  • Not ideal for small molecule or peptide antigens
Single Domain
Mouse
Mouse
  • Large spleen makes them good for monoclonal antibodies
  • Very common isotype
  • Do not need much antigen
  • Serum can be used directly in initial assays
  • Small serum volumes
  • Not ideal for short peptide antigens
Monoclonal
Rabbit
Rabbit
  • Most common host species
  • Great host for peptide and most protein antigens
  • Serum can be used directly for initial assays
  • Not ideal for highly conserved mammalian proteins
Polyclonal
Rat
Rat
  • Good for small-volume projects
  • Do not need much antigen
  • Good for anti-mouse
  • Serum can be used directly in initial assays
  • Small serum volumes
Small Volume Polyclonal

Found the information you need? Then you are ready to start creating your order. Use our Monoclonal, Polyclonal, or  Single Domain Selector Tools to begin your checkout process.For more information about ProSci's custom antibody development services, visit the Custom Antibody Services Page or fill out the following form.


 

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