Antibody Purification

ProSci Antibody Purification

 

As part of our additional services, ProSci purifies antibodies from serum, ascites fluid, culture medium, or yolks using a variety of purification procedures. These procedures include protein A purification, protein G purification, immuno-affinity, or IgY purification.

Protein A or G Purification

These proteins isolate total mammalian IgG from serum, ascites fluid, or cell culture medium. Selecting Protein A or Protein G depends on the sample’s species and antibody isotype. Protein A purification or protein G purification is not recommended for peptide antigens as antibodies against the large carrier protein will also be isolated. This method is best used with antiserum produced using protein antigens larger than 10 kDa and for purifying monoclonal antibodies from ascites or supernatant.

Immuno-Affinity

The immuno-affinity purification method isolates antigen-specific antibodies using a column constructed by coupling an antigen to a gel matrix. It is best used for isolating peptide-specific polyclonal antibodies from serum, but can also be used with protein antigens and for purifying monoclonal antibodies.

IgY Purification

This method isolates total IgY from yolks. Chicken IgY is the functional equivalent of mammalian IgG but is primarily isolated from egg yolks rather than serum. This process is performed by removing lipids first and then precipitating antibodies from the resulting solution. The total IgY can then be used “as-is” or further purified using an immuno-affinity column.

Ace Antisera   Ace Antibody
 

Before and after image of crude antiserum against a peptide antigen and immuno-affinity purified antibody using the peptide.

How it Works

Working with ProSci on antibody purification is easy: simply fill out the form or quote with some basic information, including:

  • Type of purification wanted
  • Protein being purified (ex: size, storage temperature, pH, toxicity)

One of our specialists will review the form and be in touch to confirm all of the information. Once the quote is approved, simply send in the protein to our laboratory and within 2-3 weeks, the purified protein will be sent back to your lab.

Contact Us

If you have any questions regarding our antibody purification services, please contact us at abservices@prosci-inc.com, or by calling toll-free 888-513-9525.