Antibody Labeling

ProSci Antibody Labeling

 

The use of a labeled antibody is crucial for a researcher to be able to properly identify and track their antibodies, especially if using more than one same-species antibody in a single staining experiment. ProSci is proud to feature antibody labeling in its suite of custom antibody services.

Types of Labeling Services

We offer several different types of antibody labeling protocols, depending on your needs:

  • Biotin and its reaction with streptavidin is a common lab technique used for protein and nucleic acid detection and purification. Streptavidin can bind up to four biotin molecules and can subsequently be conjugated to an antibody labeled with an avidin-biotin complex that can easily be detected. The biotin is stable and rarely interferes with the molecule that it is labeling, making it an ideal antibody labeling process when sensitive assays are needed.
  • Horseradish Peroxidase, or HRP, is commonly used to conjugate proteins in Western blots, immunohistochemistry and ELISA protocols. The HRP molecule can be linked to any biomolecule containing an available amine group.
  • Fluorescein isothiocyanate, or FITC, is a type of fluorescein that is used for biological research for bioconfugate, primary antibody, and secondary antibody labeling. They can be used to conjugate with amine, thiol, aldehyde, ketone, phosphate and carboxylate groups, depending on your needs.

How it Works

Our antibody labeling protocol is very simple and intuitive:

  1. Fill out the form for a quote and to verify the labeled antibody requirements for the service.
  2. Send in the antibody once the quote and requirements are approved.
  3. We will run the antibody through a column for labeling.
  4. Once labeling is done we will send you the antibody labeled according to your needs, as well as an official certificate reporting the results of labeling.
  5. After we receive the antibody, the labeled product is typically available around 2-3 weeks later.

Contact Us

Get in touch with us today to receive more information and a consultation about labeling antibodies. Simply fill out this form, or call our laboratory directly at (888) 513-9525.