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Custom Antibody Production
ProSci Incorporated has produced more than 10,000 custom monoclonal and custom polyclonal antibodies for researchers worldwide in academia, industry, diagnostics, and pharma. Operating our own animal facility and performing immunological services in-house allow us to tailor antibody production for almost any research need. With a capacity of more than 5,000 animals, no project is too large or too small. We look forward to assisting you with any aspect of the antibody production project, from choosing the ideal type of antibody and species for antibody production, recommending protein antigen conditions, or helping design both an antibody production protocol and an antigenicity-guaranteed peptide for your custom antibody production project. ProSci has the experience and expertise to help you develop the antibodies you need for your research needs. Rabbit Antibody Production Pricing from $620*Check for pricing below on all our packages.We offer a range of services, including:
Custom Polyclonal Antibody Production:Polyclonal antibodies are one of the most widely used research reagents. They can be used for a range of research applications, including:
We offer antibody production packages that cover all scales of projects, from a single project for a researcher targeting one protein, to the long-term large scale production needed for diagnostics. Animals are placed on an initial immunization protocol; at the end of the period you have a three day screening period to evaluate the final scheduled bleeds. Animals can then be terminated at no additional cost, exsanguinated to harvest additional serum (~60mL per rabbit; $50), or placed on an extension schedule for longer-term production ($75 per month per animal for rabbit and chicken, $200 per month per animal for goat). Animals can be maintained on extension schedules for multiple years, allowing you to obtain as much anti-serum as is needed for your research. Custom Polyclonal Antibody Production Packages:We offer custom antibody production packages for client-provided antigens, single-peptide projects, peptide cocktail projects, and phospho-peptide projects. Custom Antibody Production Package 1 uses a client-provided antigen, and includes an 8-week immunization protocol in a species of your choice. We can start a project with as little as 0.25mg of your antigen, or perform a complete immunization protocol with as little as 0.5mg of antigen in two chickens. We can perform additional services (ELISAs, purifications, western blotting, etc.) for any project to assist you in evaluating the antibodies produced. Custom Antibody Production Package 2 includes complimentary antigen design, synthesis of a 15-residue peptide at >70% HPLC purity, conjugation of the peptide to a carrier protein, an 8-week immunization protocol in a species of your choice, and an ELISA to verify titers on the first production bleed; a titer of >1:5,000 is guaranteed if you use a peptide sequence we approved. Custom Antibody Production Package 3 includes complimentary antigen design, synthesis of a 15-residue peptide at >70% HPLC purity, conjugation of the peptide to a carrier protein, an 8-week immunization protocol in a species of your choice, an ELISA to verify titers on the first production bleed, construction of an immuno-affinity column using project peptide, and purification of 25mL of serum or yolk; a titer of >1:5,000 is guaranteed if you use a peptide sequence we approved. Custom Antibody Production Package 4 includes complimentary antigen design, synthesis of a 15-residue peptide at >70% HPLC purity, conjugation of the peptide to a carrier protein, an 8-week immunization protocol in a species of your choice, an ELISA to verify titers on the first production bleed, construction of an immuno-affinity column using project peptide, and purification of 200mL of serum or yolk; a titer of >1:5,000 is guaranteed if you use a peptide sequence we approved. Peptide Cocktail Antibody Production Packages include all of the services in Package 2 and Package 3 respectively, but include the synthesis, conjugation, and immunization of your choice of 2 or 3 peptides into one set of animals. This is an option that increases the likelihood of a peptide-based antibody working for multiple applications, as it doubles or triples the available number of epitopes for the animals’ immune system to respond against. It also helps to reduce overall cost, as it minimizes the number of animals needed for a particular project. Phospho-Specific Antibody Production Packages include all of the services in Package 2, 3, and 4 respectively, but include the synthesis, conjugation, and immunization of phospho peptides into a set of rabbits. Packages 2PN, 3PN, and 4PN also offer the synthesis of the corresponding non-phospho peptide sequence, used for isolating both the phospho and non-phospho antibodies for your target protein. Additional Information:Rabbit Polyclonal Custom Antibody Production:
Chicken Polyclonal Custom Antibody Production:
Goat Polyclonal Custom Antibody Production:
Custom Phospho-Specific Antibody Production:
The most common peptide modification used for custom polyclonal antibody production is the phosphorylation of amino acids. This is accomplished immunizing host animals with a short synthetic peptide containing the phosphoryl (PO3) group on the residue of interest. The generation of custom phospho-specific antibodies can ensure that the peptide mimics the natural conformation and activity found in the protein. Phosphorylation often results in functional changes in the activity of a protein, and having antibodies recognizing the phosphorylated state (as well as the corresponding non-phosphorylated state) can be integral for studying protein pathways. There are three residues which can be phosphorylated:
The process of producing custom antibodies against a peptides containing a phospho-residue is a bit more complicated than traditional peptide antibody production. The animals can produce antibodies against additional epitopes in the peptide sequence, as well as produce antibodies that do not contain the modified phospho-residue. While a pool of phospho and non-phospho antibodies can be difficult to work with, if purified from the anti-serum the non-phospho antibody can be very helpful for studying protein pathways. This service is included in our packages 3PN and 4PN.
Peptide Cocktail Custom Antibody Production:
Need a quick approach to find a good polyclonal antibody, and don’t have recombinant protein available? Producing custom polyclonal antibodies using several peptides can be a good solution. However, separate immunization protocols can become very expensive, very quickly. ProSci offers packages for synthesizing, conjugating, and immunizing multiple peptides into one set of animals. Benefits of peptide cocktail custom antibody production include:
ProSci offers four peptide cocktail custom antibody production packages, each of which provides a minimum of four antibodies against your protein of interest.
Custom Monoclonal Antibody Production:
ProSci can help you develop the monoclonal antibodies needed for your research. We understand researchers have varying needs for levels of involvement, depending on the target. We have developed packages for different levels of involvement in the project, from researcher screening (you screen positive wells following fusion for clones of interest) to ProSci sending western blot and immunocytochemistry positive final clones. Additionally, ProSci offers packages for protein and peptide antigens. ProSci’s fusion success rate for hybridoma production is greater than 95%, using techniques developed in-house. All work is performed by skilled technicians on premises in our Poway, California laboratories. Monoclonal antibody production packages can be scaled up to almost any magnitude. We can screen against multiple antigens (additional charges apply) to develop the specific clone you need. ProSci has repeatedly proven our abilities to produce monoclonal antibodies against difficult targets. We use 5 Balb/c mice as a production strain for monoclonal antibody development. Balb/c is a very common species used throughout the industry for monoclonal antibody development due to their large spleen to body size ratio. This yields a higher number of spleenocytes at fusion. Each of our monoclonal antibody production packages includes:
Ascites Production:ProSci can produce antibody from a Balb/c-derived cell line through the production of ascites fluid. This is done by injecting the cell line into the primed peritoneal cavity of Balb/c mice, and harvesting the fluid. We can do this from a cell line produced through our hybridoma development programs, or from a cell line you send to us. Our ascites production includes:
Peptide Synthesis:ProSci can synthesize almost any custom peptide for your needs. We provide a high quality product in a fast timeline; this is our commitment to our clients.
Antigen Design Assistance:ProSci offers free antigen design assistance for peptide antigens. If you provide us with the sequence (or protein accession number) our scientists will analyze your protein and select the regions which offer the best chance of eliciting an immune response.
* 2 rabbit polyclonal antibody production with client supplied antigen |
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