Datasheet

CCR5 Antibody
CATALOG NUMBER: 1112

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:CCR5 antibody can be used for Western blot at 1 μg/mL. CCR5 antibody can also detect CCR5 by immunohistochemistry at 2.5 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1208 - THP-1 Cell Lysate
 2) Cat. No. 1369 - Human Lymph node Tissue Lysate
 3) Cat. No. 11-521 - Human Lymphoid Tissue Tissue Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 39 kDa

Observed: 40 kDa
IMMUNOGEN:CCR5 antibody was raised against a 15 amino acid peptide near the amino terminus of human CCR5.

The immunogen is located within the first 50 amino acids of CCR5.
HOST SPECIES:Rabbit

Properties

PURIFICATION:CCR5 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:CCR5 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:CCR5 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:CCR5 Antibody: CKR5, CCR-5, CD195, CKR-5, CCCKR5, CMKBR5, IDDM22, CC-CKR-5, C-C chemokine receptor type 5, CHEMR13, C-C CKR-5
ACCESSION NO.:AAB57793
PROTEIN GI NO.:2104520
OFFICIAL SYMBOL:CCR5
GENE ID:1234

Background

BACKGROUND:CCR5 Antibody: Human immunodeficiency virus (HIV) and related virus require coreceptors, in addition to CD4, to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors. Among them, CCR5 (CC-CKR-5) is a principal coreceptor for macrophage- and dual-tropic HIV-1 strains fusion and entry of human white blood cells. CCR5 is required for the infection by HIV-1, HIV-2, and SIV. The beta-chemokines RANTES, MIP-alpha and MIP-beta are the ligands for CCR5 and prevent infection by M-tropic HIV-1. CXC5 associates with the surface CD4-gp120 of HIV complex and leads to membrane fusion and virus entry of target cells. The amino-terminal domain and the extracellular loops of CCR5 serve as HIV biding sites. CCR5 messenger RNA is expressed in lymphoid organs and monocytes.
REFERENCES: 1) Dimitrov, DS. Cell 1997; 91:721-30
2) Littman, DR. Cell 1998; 93:677-80
3) Deng, H, et al. Nature 1996; 381:661-6
4) Dragic, T, et al. Nature 1996; 381:667-73

For Research Use Only