Datasheet

CAD Antibody
CATALOG NUMBER: 2011

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:M
TESTED APPLICATIONS:E, WB, IHC, IF
APPLICATIONS:CAD antibody antibody can be used for detection of CAD by Western blot at 0.5 μg/mL. A 40 kDa band should be detected. Antibody can also be used for immunohistochemistry starting at 1 μg/mL. For immunofluorescence start at 10 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1402 - Mouse Lung Tissue Lysate
 2) Cat. No. 1405 - Mouse Kidney Tissue Lysate
PREDICTED MOLECULAR WEIGHT:40 kDa
IMMUNOGEN:CAD antibody was raised against a 16 amino acid peptide near the carboxy terminus of murine CAD.
HOST SPECIES:Rabbit

Properties

PURIFICATION:CAD Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:CAD Antibody is supplied in PBS containing 0.02% sodium azide.
STORAGE CONDITIONS:CAD antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:CAD Antibody: CAD, CPAN, 40kDa, DFF40, Didff, 5730477D02Rik, Cad, DNA fragmentation factor subunit beta, Caspase-activated deoxyribonuclease, CAD
ACCESSION NO.:O54788
PROTEIN GI NO.:20137695

Background

BACKGROUND
CAD Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD (for caspase activated deoxyribonuclease). The human homologue of mouse CAD was more recently identified by two groups independently and termed CPAN and DFF40. Human DFF45 and its mouse homologue ICAD are the inhibitors of CPAN/DFF40 and CAD, respectively. Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei. Activation of CAD/DFF40, which causes DNA degradation, is the hallmark of apoptotic cell death.
REFERENCES
1) Enari M, Sakahira H, Yokoyama H, Okawa K, Iwamatsu A, Nagata S. A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD. Nature 1998;391:43-50
2) Sakahira H, Enari M, Nagata S. Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis. Nature 1998;391:96-99
3) Liu X, Li P, Widlak P, Zou H, Luo X, Garrard WT, Wang X The 40-kDa subunit of DNA fragmentation factor induces DNA fragmentation and chromatin condensation during apoptosis. Proc Natl Acad Sci USA 1998;95:8461-6
4) Halenbeck R, MacDonald H, Roulston A, Chen TT, Conroy L, Williams LT. CPAN, a human nuclease regulated by the caspase-sensitive inhibitor DFF45. Curr Biol 1998;8:537-40

For Research Use Only