FormulationRabbit polyclonal immunoglobulin in Dulbecco's phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol, with 1.0 mg/mL BSA (IgG, protease free) as a carrier.
Clonality/CloneThis is a polyclonal antibody.
SpecificityHuman and mouse c-Fos. Rat c-Fos (100% homologous) has not been tested, but is expected to react.
Sourcec-Fos antibody was produced against a chemically synthesized phosphopeptide derived from the region of human c-Fos that contains threonine 325. The sequence is conserved in human, mouse and rat.
Purificationc-Fos antibody was purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated c-Fos. The final product is generated by affinity chromatography using a c-Fos-derived peptide that is phosphorylated at threonine 325.
Hostc-Fos [pT325] antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
ApplicationFor Western blotting applications, we recommend using the antibody at a 1:1000 dilution.
BufferRabbit polyclonal immunoglobulin in Dulbecco's phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol, with 1.0 mg/mL BSA (IgG, protease free) as a carrier.
StorageStore at -20˚C. We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20˚C. For shipment or short-term storage (up to one week), 2 - 8˚C is sufficient.
- Human epidermoid carcinoma (A431) cells stimulated with EGF or 15% serum.]
- Monje, P., et al. (2003) Phosphorylation of the carboxyl-terminal transactivation domain of c-Fos by extracellular signal-regulated kinase mediates the transcriptional activation of AP-1 and cellular transformation induced by platelet-derived growth factor. Mol. Cell. Biol. 23(19):7030-7043.
- Wang, L.H., et al. (2003) The cis decoy against the estrogen response element suppresses breast cancer cells via target disrupting c-fos not mitogen-activated protein kinase activity. Cancer Res. 63(9):2046-2051.
- Deng, T. and M. Karin (1994) c-Fos transcriptional activity stimulated by H-Ras-activated protein kinase distinct from JNK and ERK. Nature 371(6493):171-175.
- Lu, Y., et al. (2004) Overexpression of ErbB2 receptor inhibits IGF-I-induced Shc-MAPK signaling pathway in breast cancer cells. Biochem. Biophys. Res. Commun. 313(3):709-715.]