c-Fos [pT232] glyc Antibody | catalog# XBP-4056

Background

c-Fos is a ~60 kDa immediate early gene product and a member of AP-1 family. c-Fos dimerizes with c-Jun to form the transcriptionally active complex AP-1, and functions in regulating cell proliferation and differentiation. c-Fos expression and activation are induced in many tumors (including breast cancer), and in response to a variety of stimuli (including growth factors, cytokines and stress). ERK1&2 mediate growth factor-induced c-Fos induction and transcripional activation by directly phosphorylating the carboxyl terminal domain of c-Fos. c-Fos is phosphorylated by Ras on threonine 232, the homologue of serine 73 of c-Jun, which results in augmenting its transcriptional activity.

Description

Lysates prepared from SKBR3 cells were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 3% Milk-TBST buffer for one hour at room temperature, and incubated with c-Fos [pT232] antibody for two hours at room temperature in a 3% Milk-TBST buffer, following prior incubation with: no peptide (1), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphothreonine-containing peptide (3), or, the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab')2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal™ method.