Datasheet

BAFF Antibody
CATALOG NUMBER: 2221

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, ICC, IF, WB
APPLICATIONS:BAFF antibody can be used for detection of BAFF by Western blot a 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1209 - HL60 Cell Lysate
 2) Cat. No. 1406 - Mouse Spleen Tissue Lysate
 3) Cat. No. 17-009 - HL-60 Cell Slide
PREDICTED MOLECULAR WEIGHT:31 kDa
IMMUNOGEN:BAFF antibody was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human BAFF.

The immunogen is located within the last 50 amino acids of BAFF.
HOST SPECIES:Rabbit

Properties

PURIFICATION:BAFF Antibody is Ion exchange chromatography purified.
PHYSICAL STATE:Liquid
BUFFER:BAFF Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:BAFF antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:BAFF Antibody: DTL, BAFF, BLYS, CD257, TALL1, THANK, ZTNF4, TALL-1, TNFSF20, UNQ401/PRO738, Tumor necrosis factor ligand superfamily member 13B, B lymphocyte stimulator
ACCESSION NO.:NP_006564
PROTEIN GI NO.:5730097
OFFICIAL SYMBOL:TNFSF13B
GENE ID:10673

Background

BACKGROUND:BAFF Antibody: Members in the TNF superfamily regulate immune responses and induce apoptosis. A novel member in the TNF family was recently identified by several groups and designated BAFF (for B cell Activating Factor belonging to the TNF Family), BLyS (for B Lymphocyte Stimulator), TALL-1 (for TNF- and ApoL-related Leukocyte-expressed Ligand), and THANK (for TNF Homologue that Activate Apoptosis, NF-κB and c-jun N-terminal Kinase). BAFF/BLyS was characterized as a B cell activator since it induced B cell proliferation and immunoglobulin secretion. Three receptors for BAFF were recently identified and designated TACI, BCMA and BAFF-R. BAFF and its receptors are essential for B cell development, survival, and humoral immune responses. BAFF is involved in the development of autoimmune diseases including systemic lupus erythaematosus and rheumatoid arthritis.
REFERENCES: 1) Moore PA , Belvedere O, Orr A, et al. BLyS: member of the tumor necrosis factor family and B lymphocyte stimulator. Science 1999;285:260-3
2) Schneider P, MacKay F, Steiner V, et al. BAFF, a novel ligand of the tumor necrosis factor family, stimulates B cell growth. J Exp Med 1999;189:1747-56
3) Shu HB, Hu WH, Johnson H. TALL-1 is a novel member of the TNF family that is down-regulated by mitogens. J Leukoc Biol 1999;65:680-3
4) Mukhopadhyay A, Ni J, Zhai Y, Yu GL, Aggarwal BB. Identification and characterization of a novel cytokine, THANK, a TNF homologue that activates apoptosis, nuclear factor-κB, and c-Jun NH2-terminal kinase. J Biol Chem 1999 ;274:15978-81

For Research Use Only