Datasheet

BACE2 Antibody
CATALOG NUMBER: 2249

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Rat: (100%), Mouse: (100%)
TESTED APPLICATIONS:ELISA, IHC-P, WB
APPLICATIONS:BACE2 antibody can be used for detection of BACE2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1301 - Human Heart Tissue Lysate
 2) Cat. No. 1461 - Rat Heart Tissue Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 57 kDa

Observed: 57 kDa
IMMUNOGEN:BACE2 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human BACE2.

The immunogen is located within the last 50 amino acids of BACE2.
HOST SPECIES:Rabbit

Properties

PURIFICATION:BACE2 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:BACE2 Antibody is supplied in PBS containing 0.02% sodium azide.
STORAGE CONDITIONS:BACE2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:BACE2 Antibody: ASP1, BAE2, DRAP, AEPLC, ALP56, ASP21, CDA13, CEAP1, UNQ418/PRO852, Beta-secretase 2, Aspartic-like protease 56 kDa, ASP1
ACCESSION NO.:AAF17078
PROTEIN GI NO.:6561812
OFFICIAL SYMBOL:BACE2
GENE ID:25825

Background

BACKGROUND:BACE2 Antibody: Accumulation of the amyloid-beta (Abeta) plaque in the cerebral cortex is a critical event in the pathogenesis of Alzheimer's disease. Abeta peptide is generated by proteolytic cleavage of the beta-amyloid protein precursor (APP) at beta- and gamma-sites by proteases. The long-sought beta-secretase was recently identified by several groups independently and designated beta-site APP cleaving enzyme (BACE) and aspartyl protease 2 (Asp2). BACE/Asp2 is a novel transmembrane aspartic protease and co-localizes with APP. A BACE homolog was recently cloned and designated BACE2, Asp1, DRAP (for Down region aspartic protease), and memapsin 1. BACE2 also cleaves APP at b-site and at a different site within Abeta. BACE2 locates on chromosome 21q22.3, the so-called ‘Down critical region', suggesting that BACE2 and Abeta may also contribute to the pathogenesis of Down syndrome.
REFERENCES: 1) Vassar R, et al. β-secretase cleavage of Alzheimer's amyloid precursor protein by the transmembrane aspartic protease BACE. Science 1999;286:735-41
2) Hussain I, et al. Identification of a novel aspartic protease (Asp 2) as β-secretase. Mol Cell Neurosci 1999;14:419-27
3) Sinha S, et al. Purification and cloning of amyloid precursor protein β-secretase from human brain. Nature 1999;402:537-40
4) Yan R, et al. Membrane-anchored aspartyl protease with Alzheimer's disease β-secretase activity. Nature 1999;402:533-7

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