Datasheet

B2M Antibody
CATALOG NUMBER: 7439

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Mouse: (83%), Rat: (83%)
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:B2M antibody can be used for detection of B2M by Western blot at 1 - 2 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1220 - SK-N-SH Cell Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 13 kDa

Observed: 12 kDa
SPECIFICITY:B2M antibody is human reactive.
IMMUNOGEN:B2M antibody was raised against a 15 amino acid peptide near the carboxy terminus of human B2M.

The immunogen is located within the last 50 amino acids of B2M.
HOST SPECIES:Rabbit

Properties

PURIFICATION:B2M Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:B2M Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:B2M antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:B2M Antibody: Beta-2-microglobulin
ACCESSION NO.:NP_004039
PROTEIN GI NO.:4757826
OFFICIAL SYMBOL:B2M
GENE ID:567

Background

BACKGROUND:B2M Antibody: Beta2-microglobulin (B2M) is a principal component of the Major Histocompatibility Complex (MHC) class I molecule, a ternary membrane protein complex that displays fragments derived from proteolyzed cytosolic proteins on the surface of cells for recognition by the surveillance immune system (1,2). B2M is involved in the presentation of peptide antigens to the immune system and plays a critically important role in immune system function (3). It is expressed on nearly all nucleated cells and contains one Ig-like C1-type (immunoglobulin-like) domain (2,3). Mutations in the Beta 2-microglobulin gene can enhance the progression of malignant melanoma and osteoarthropathy (4,5).
REFERENCES: 1) Krangel MS, Orr HT, and Strominger JL. Assembly and maturation of HLA-A and HLA-B antigens in vivo. Cell 1979; 18:979-91.
2) Skjodt K, Welinder KG, Crone M, et al. Isolation and characterization of chicken and turkey beta 2-microglobulin. Mol. Immunol. 1986; 23:1301-9.
3) Ohashi K. Pathogenesis of beta2-microglobulin amyloidosis. Pathol. Int. 2001; 51:1-10.
4) Blum C, Graham A, Yousefzadeh M, et al. The expression ratio of Map7/B2M is prognostic for survival in patients with stage II colon cancer. Int. J. Oncol. 2008; 33:579-84.

For Research Use Only