Datasheet

B-NGF Antibody
CATALOG NUMBER: XP-5110

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:H
TESTED APPLICATIONS:E, WB, Neut
APPLICATIONS:Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hβ-NGF (1 ng/mL), a concentration of 0.05-0.08 μg/mL of this antibody is required.

ELISA:

To detect hβ-NGF by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2-0.4 ng/well of recombinant hβ-NGF.

Western Blot:
To detect hβ-NGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hβ-NGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
USER NOTE:Centrifuge vial prior to opening.
IMMUNOGEN:Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hβ-NGF (Human beta-Nerve Growth Factor).
HOST SPECIES:Rabbit

Properties

PURIFICATION:Anti-hβ-NGF specific antibody was purified by affinity chromatography employing immobilized hβ-NGF matrix.
PHYSICAL STATE:Powder
CONCENTRATION:Lyophilized
STORAGE CONDITIONS:B-NGF antibody is stable for at least 2 years from date of receipt at-20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at-20˚C. Avoid repeated freeze-thaw cycles.
CLONALITY:This is a polyclonal antibody.
CONJUGATE:None

Additional Info

ALTERNATE NAMES:NGFB, HSAN5, Beta-NGF, NGFB, Beta-nerve growth factorBeta-NGF
ACCESSION NO.:P01138
PROTEIN GI NO.:90110037

Background

For Research Use Only