Datasheet

AXIN2 Antibody
CATALOG NUMBER: 6163

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Chicken: (85%)
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:AXIN2 antibody can be used for detection of AXIN2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1402 - Mouse Lung Tissue Lysate
PREDICTED MOLECULAR WEIGHT:85, 93 kDa
SPECIFICITY:AXIN2 antibody is predicted to not cross-react with AXIN1. At least four isoforms of AXIN2 are known to exist; this antibody will recognize all four.
IMMUNOGEN:AXIN2 antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of human AXIN2.

The immunogen is located within amino acids 780 - 830 of AXIN2.
HOST SPECIES:Rabbit

Properties

PURIFICATION:AXIN2 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:AXIN2 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:AXIN2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:AXIN2 Antibody: AXIL, ODCRCS, Axin-2, Axin-like protein, Axil
ACCESSION NO.:AAF22799
PROTEIN GI NO.:195927059
OFFICIAL SYMBOL:AXIN2
GENE ID:8313

Background

BACKGROUND:AXIN2 Antibody: Like the related protein AXIN1, AXIN2 is thought to play an important role in the regulation of the stability of beta-catenin in the Wnt signaling pathway. In mouse, AXIN2 organizes a multiprotein complex of APC (adenomatous polyposis of the colon), beta-catenin, glycogen synthase kinase 3-beta, and AXIN2, which leads to the degradation of beta-catenin, which is thought to be an important event in the genesis of a number of malignancies. The AXIN2 gene has been mapped to 17q23-q24, a region that shows frequent loss of heterozygosity in breast cancer, neuroblastoma, and other tumors. Mutations in this gene have been associated with colorectal cancer with defective mismatch repair.
REFERENCES: 1) Katoh M and Katoh M. WNT signaling pathway and stem cell signaling network. Clin. Cancer Res.2007; 13:4042-5.
2) Behrens J, Jerchow BA, Wurtele M, et al. Functional interaction of an axin homolog, conductin, with beta-catenin, APC, and GSK3beta. Science1998; 280:596-9.
3) Hadjihannas MV and Behrens J. CIN by WNT: growth pathways, mitotic control and chromosome instability in cancer. Cell Cycle2006; 5:2077-81.
4) Mai M, Qian C, Yokomizo A, et al. Cloning of the human homolog of conductin (AXIN2), a gene mapping to chromosome 17q23-q24. Genomics1999; 55:341-4.

For Research Use Only