Datasheet

AXIN1 Antibody
CATALOG NUMBER: 6005

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Chicken: (79%)
TESTED APPLICATIONS:ELISA, IF, WB
APPLICATIONS:AXIN1 antibody can be used for detection of AXIN1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunoflourescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1220 - SK-N-SH Cell Lysate
 2) Cat. No. 10-301 - Human Brain Tissue Slide
IMMUNOGEN:AXIN1 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human AXIN1.

The immunogen is located within amino acids 620 - 670 of AXIN1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:AXIN1 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:AXIN1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:AXIN1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:AXIN1 Antibody: AXIN, PPP1R49, AXIN, Axin-1, Axis inhibition protein 1, hAxin
ACCESSION NO.:AAC51624
PROTEIN GI NO.:2252820
OFFICIAL SYMBOL:AXIN1
GENE ID:8312

Background

BACKGROUND:AXIN1 Antibody: AXIN1 is a cytoplasmic protein which contains a regulation of G-protein signaling (RGS) domain and a dishevelled and axin (DIX) domain and is thought to function as a negative regulator of the WNT signaling pathway that regulates embryonic axis formation. AXIN1 interacts with adenomatosis polyposis coli (APC), beta-catenin, glycogen synthase kinase 3 beta, forming a tetrameric complex resulting in the regulation of the stabilization of beta-catenin. Mutations in the AXIN1 gene have been associated various carcinomas, indicating that it also functions as a tumor suppressor.
REFERENCES: 1) Zeng L, Fagotto F, Zhang T, et al. The mouse Fused locus encodes Axin, an inhibitor of the Wnt signaling pathway that regulates embryonic axis formation. Cell1997; 90:181-92.
2) Kishida S, Yamamoto H, Ikeda S, et al. Axin, a negative regulator of the wnt signaling pathway, directly interacts with adenomatous polyposis coli and regulates the stabilization of beta-catenin. J. Biol. Chem.1998; 273:10823-6.
3) Nakamura T, Hamada F, Ishidate T, et al. Axin, an inhibitor of the Wnt signaling pathway, interacts with beta-catenin, GSK-3beta and APC and reduces the beta-catenin level. Genes Cells1998; 3:395-403.
4) Salahshor S and Woodgett JR. The links between axin and carcinogenesis. J. Clin. Pathol.2005; 58:225-36.

For Research Use Only