Datasheet

ATP2C1 Antibody
CATALOG NUMBER: 6287

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (94%), Rat: (86%)
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:ATP2C1 antibody can be used for detection of ATP2C1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1403 - Mouse Brain Tissue Lysate
SPECIFICITY:At least four isoforms of ATP2C1 are known to exist; this antibody will recognize only the three longest isoforms. ATP2C1 antibody will not cross-react with ATP2C2.
IMMUNOGEN:ATP2C1 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human ATP2C1.

The immunogen is located within the last 50 amino acids of ATP2C1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:ATP2C1 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:ATP2C1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:ATP2C1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:ATP2C1 Antibody: HHD, BCPM, PMR1, SPCA1, hSPCA1, ATP2C1A, KIAA1347, PMR1L, HUSSY-28, Calcium-transporting ATPase type 2C member 1, ATPase 2C1
ACCESSION NO.:NP_001001486
PROTEIN GI NO.:312836765
OFFICIAL SYMBOL:ATP2C1
GENE ID:27032

Background

BACKGROUND:ATP2C1 Antibody: ATP2C1, also known as secretory pathway Ca2+/Mn2+-ATPase (SPCA) 1, belongs to the family of P-type cation transport ATPases. This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of the calcium from the cytosol to the Golgi lumen. Defects in this gene cause Hailey-Hailey disease, an autosomal dominant disorder characterized by persistent blisters and erosions of the skin. Unlike the related protein ATP2C2, ATP2C1 is ubiquitously expressed and displays a lower maximal turnover rate for overall Ca2+-ATPase reaction and a higher apparent affinity for cytosolic Ca2+ activation of phosphorylation. Recent evidence suggests that ATP2C1 is a key regulator of insulin-like growth factor receptor (IGF1R) processing in tumor progression in basal breast cancers.
REFERENCES: 1) Hu Z, Bonifas JM, Beech J, et al. Mutations in ATP2C1, encoding a calcium pump, cause Hailey-Hailey disease. Nat. Genet. 2000; 24:61-5
2) Dode L, Andersen JP, Vanoevelen J, et al. Dissection of the functional differences between human secretory pathway Ca2+/Mn2+-ATPase (SPCA) 1 and 2 isoenzymes by steady-state and transient kinetic analyses. J. Biol. Chem. 281:3182-9.
3) Grice DM, Vetter I, Faddy HM, et al. Golgi calcium pump secretory pathway calcium ATPase 1 (SPCA1) is a key regulator of insulin-like growth factor receptor (IGF1R) processing in the basal-like breast cancer cell line MDA-MB-231. J. Biol. Chem. 2010; 285:37458-66

For Research Use Only